Cytology and Histology Flashcards
Cytology
study of the cell
What cell can be seen with the human eye?
human egg cell
Common Structural and Functional Features of the Cell
organelles, plasma membrane, cytoplasm (fluid inside cell)
Zygote
formed when a sperm fertilizes an egg to make one cell with complete set of genetic material
Cells…
structural building blocks of all life, produced by the division of preexisting cells, form all the structures in the body cells, perform all vital functions of the body
Cells perform all the functions of living things
grow, develop, reproduce, take in energy and use, produce waste, respond to stimulus, maintain constant internal conditions, move, communicate
Sex Cells
sperm and oocytes
How much genetic material do sperm/oocytes contain?
half
Somatic Cells
all the other cells in the body that are not sex cells
3 Main Parts of the Cell
plasma membrane, cytoplasm, phospholipid bilayer
Plasma Membrane
outer layer of the cell made by phospholipid bilayer
Cytoplasm
everything inside the membrane
Cytoplasm includes…
cytosol and organelles
Cytosol
fluid inside of the cell
Organelles
specialized structures which help carry out function in cell
Organelles float in the
cytosol
Nonmembranous organelles
cytoskeleton, cilia, flagella, microvilli, ribosomes
Membranous organelles
mitochondria, nucleus, endoplasmic reticulum, golgi apparatus, lysosomes, peroxisomes
Semipermeable
allows certain materials to travel through the cell
Extracellular
outside of the phospholipid
Intracellular
inside of the phospholipid
4 components of the cell membrane
phospholipid bilayer, proteins, cholesterol, carbohydrates
Purpose of proteins in cell membrane
provide structure, form channels, act as receptor sites
Leak channel/pore
lets different compounds leak through
Gated channel
lets certain compounds go through
Cholesterol
stabilizes cell membrane and fluidity
Carbohydrates
helps other cells identify the cell (found on the outside of the cell)
Membrane Transport types
passive and active processes
Passive Process
moving from high concentration to low
Active Processes
moving from low to high concentration; requires work
Types of passive processes
diffusion, osmosis, facilitated diffusion
Types of active processes
pump, bulk transport
Diffusion
movement of molecules from high to low concentration
Osmosis
water traveling across a membrane
Facilitated diffusion
molecules that are too large or too much of a charge so they use protein to go across
Facilitated diffusion proteins
protein channel or carrier protein
Pump transport
proteins that can move specific substances across membrane
Most common pump transport
sodium potassium exchange pump
Sodium potassium exchange pump products
pushed out 3 sodium ions per ATP and pulls in 2 potassium ions
Bulk transport
uses special membrane bubbles (vesicles) to transport large particles into/out of cell
Types of bulk transport
endocytosis and exocytosis
Endocytosis
bring into the cell
Types of endocytosis
phagocytosis, pinocytosis
Phagocytosis
membrane envelops cell (cell eating)
Pinocytosis
vesicle that forms at plasma membrane bringing fluid into cell (fluid drinking)
Exocytosis
waste is removed in vesicles out the cell
Cytoskeleton
proteins that give the cell structure
Cytoskeleton helps…
shape, support and move things around in the cell
Cytoskeleton is organized into
filaments and tubules
3 different types of filaments/tubules
microtubule, microfilaments, intermediate filament
Microtubule
maintain the structure of cell, helps with cellular division, moves/anchors organelles, make up cilia and flagella
Microfilament
causes shape of cell to change
Intermediate filament
give structure to cell: stabilize organelle position, provide strength
Cell extensions
cilia, flagella, microvilli
cilia provides… (bacteria)
movement
cilia provides… (human cells)
move things along outer surface
cilia physical appearance
numerous and short
where is cilia found?
uterus and respiratory tract
flagella
grouping of microtubules that help to move the cell or move things over the surface of the cell
only human cell with flagella
sperm cell; one long extension which helps cell swim
flagella physical appearance
few and long appendage
Microvilli
microextension of the cell produced by the plasma membrane
purpose of microvilli
increases surface area; allows increased levels of absorption from outside the cell
where is microvilli found?
found in high absorption areas: small intestine
ribosome
where protein synthesis occurs
where are ribosomes found?
cytoplasm and rough ER
free ribosome
found open in cytoplasm
free ribosomes make proteins used for…
the inside of the cell
fixed ribosomes
attached to ER
fixed ribosomes make proteins that…
cell needs and exported from the cell for work elsewhere in the body
ribosomes are composed of
rRNA and proteins
Mitochondria
produces ATP
compartments of the mitochondria
2 compartments: inner and outer
positives of 2 compartments in mitochondria
provides the ability to have 2 different chemical reactions
what does the mitochondria burn to release energy?
hydrocarbons (glucose)
Cells which perform more work have…
more mitochondria (arms, legs, heart muscles)
nucleus
control center of the cells which contains DNA
DNA
genetic material of the cell
largest organelle of the cell
nucleus
nucleus is surrounded by
nuclear envelope
nuclear envelope
double layered membrane
nuclear pores
holes in membrane which allows RNA through
DNA floating in the nucleus is transcribed into
RNA and travels out to ribosomes
endoplasmic reticulum (ER)
network of membranes throughout the cytoplasm where products are synthesized or stored
smooth ER
synthesizes lipids, stores calcium ions, and plays role in detoxification
detoxification
removes harmful substances from the cell
rough ER
synthesizes proteins; where fixed ribosomes are
proteins are made of
amino acids strung together
golgi apparatus
responsible for packaging proteins in vesicles for use outside of cell, repairs/renews/modifies plasma membrane
Lysosome and peroxisomes
small vesicles that contain digestive enzymes; responsible for absorbing nutrients (good at digesting fatty acids)
lysosomes
break down excess or worn down organelles using enzymes contained in them, destroy invading pathogens
lysosome enzymes work in
oxygen poor and low pH/acidic environment
peroxisomes hold onto enzymes which require
oxygen
peroxisomes
digestive enzymes surrounded my membrane
lysosomes/peroxisomes can form
solids cores; high density of enzyme
solid core in peroxisome
crystalloid core
enzymes
proteins which cause a reaction to occur
3 main forms of cell attachment
tight junction, desmosomes, gap junction
tight junction (impermeable junctions)
prevents leaking between cells and waterproof; prevents molecules from passing through intercellular space using interlocking junctional proteins
desmosomes (strong anchoring junctions)
bind adjacent cells to help form an internal tension reducing network of fibers
desmosomes structure
proteins extend into the cytoplasm of adjacent cells and interlock into intracellular space between the two membranes
gap junctions (communication junctions)
allow for ions and small molecules to pass through allowing intracellular communication
gap junction communication
Particles that pass through relay a chemical message from one cell to another cell; allows heart cells to beat at the same time
gap junction are made of proteins that form
channels between membranes
desmosomes help reinforce
tight junctions and gap junctions; always found with desmosomes
which junction can be found alone?
desmosomes
simple squamous ET purpose
protection and allows diffusion
simple squamous ET found in
lungs, kidney
stratified squamous ET purpose
protection (physical, chemical, infection)
epithelial tissue characteristics
- cellularity 2. polarity 3. attachment 4. regeneration 5. avascular
basement membrane
network of glycoproteins and protein providing attachment site (apical and basal surface)
exocrine glands
release secretion directly onto body’s external or internal surfaces
3 classifications of exocrine glands
merocrine gland, apocrine gland, holocrine glands
most common exocrine gland
merocrine gland
merocrine gland
secrete product through exocytosis; released in duct
what kind of glands are included in merocrine gland?
sweat, salivary, gastric glands
apocrine gland
release secretion by pinching off the entire top of the cell
what kind of glands are included in apocrine gland?
mammary glands, ceruminous (ear wax glands)
holocrine gland
entire cell ruptures and dies which releases the product into the duct; cell replacement undergoes
what kind of glands are included in holocrine gland?
sebaceous oil glands
connective tissue characteristics
- no cellularity 2. no polarity 3. no attachment 4. slow regeneration 5. vascular
matrix
ground substance; everything outside the cell
chondrocytes
cells that live within cartilage; responsible for secreting polysaccharides
chondroitin sulfates
polysaccharides that form semi-solid gel with matrix proteins to make gel of proteoglycans
lacuna(e)
space created in supportive CT (cartilages)
proteoglycans
mix of proteins and carbohydrates
weakest supportive CT
hyaline cartilage CT (does not have anchoring fibers)
cells present in hyaline cartilage
chondrocytes, collagen fibers
hyaline cartilage
flexible support/protection, reduce friciton
hyaline cartilage location
long bonds, ribs, nose, respiratory tract, embryonic skeleton
strongest cartilage
fibrocartilage CT
fibrocartilage CT
limit bone to bone contact and limits joint motions (spine vertebrae, meniscus of knee)
most flexible CT
elastic cartilage CT
elastic cartilage CT
support, maintain shape (flexibility)
elastic cartilage CT location
pinna of ear, epiglottis, auditory tubes, tip of nose
bone CT
site of active blood cell formation, mineral/energy storage, levels for body movement, supportive framework
hydroxyapatite
minerals which make up hardened bone matrix
what do minerals in hydroxyapatite allow for?
resistance to compression and provides hardness
what do the minerals form?
calcium crystals
cells present in bone CT
osteocyte, osteoblast, osteogenic cell, osteoclast
bone cell maturation
osteogenic cells can mature into osteoblasts which can mature into osteocytes
osteogenic cell
stem cells that can develop into active bone cells (osteoblasts)
where are osteogenic cells present?
outside of bone, along the outside surface, along inner surface
osteocyte
mature bone cells which maintain bone tissue
spongey v compact bone
compact bone: dense and solid
spongy bone: open
ectoderm (outer layer)
forms exterior surfaces (eg. epidermal cells of skin, epidermal derivatives: hair/nails/glands, neural components: neurons/brain/spinal cord/nerves, neural crest cells)
mesoderm (middle layer)
develops into the internal epithelial structures (eg. lining of body cavities, lining of kidney tubules, notochord, bone tissue, tubule cell of the kidney, red blood cells, facial muscle)
endoderm (internal layer)
glandular derivatives of the digestive, respiratory, urinary, reproductive, endocrine systems; form functional part of glandular part of organs
CT proper made of
cells, ground substance, protein fibers
CT proper tissue
elastic and collagen
dense irregular v regular
regular movement is linear, irregular movement is bilateral