Culturing Microorganism Required Practical

Question 1

where do we grow bacteria

Answer 1

agar plate, contains lots of nutrients

Question 2

what is the aseptic technique & examples

Answer 2

• preventing contamination
• sterlilising equipment (passing innoculation loop through bunsen burner flame ect)
• opening agar plate lid at an angle

Question 3

what is the method for testing the effect of different antibiotics on bacteria

Answer 3

1. clean surfaces with disinfectant, this kills microorganisms that could contaminate the culture
2. sterilise innoculating loop by passing it through a bunsen burner flame
3. open agar gel plate at an angle
4. us loop to spread a bacteria evenly over the plate, be gentle to ensure you don’t break the agar
5. place sterile filter paper discs conatining different antibiotics onto the plate
6. place tape on either side of the lid to secure it
7. incubate the plate at 25c