CRYO-LAB & LN2

Question 1

Nitrogen will vaporize during any manipulation of cryo dewars or samples

Answer 1

true

Question 2

One Liter of Nitrogen Produce………../L of gas when heated to Room Temperature

Answer 2

601 L of gas

Question 3

in cryo-storge room, the risk of Anoxia can be influenced by

1. The size of the room
2. The ventilation in the room
3. The cryo activity in the room

Answer 3

all true

Question 4

What are the main risks from working with cryogenic material?

Answer 4

Loss of consciousness or death from lack of oxygen

Burns from splashing or direct contact

Question 5

main precautions to minimize the risks of Anoxia in cryo-storage area?

Answer 5

presence of sufficient ventilation

oxygen detectors

close availability of Individual portable respirators

Question 6

What are the precautions should you take to make the cryo-lab safer?

Answer 6

-Display warning signs of low temperature and low O2

-Make it mandatory for all staff to:
-read the users manual
-wear protective gloves and related equipment
-wear face and eye protective equipment at all times.
- always adequate ventilation& fresh air
-Warn of risk in touching any equipment that has been in contact with cryogenic equipment

Question 7

General precautions in Cryo-Lab

Answer 7

The Unprotected hand should never manipulate any item that has been in contact with cryogenic material

Even if you are wearing the correct safety gloves, you should never submerge your hands in cryogenic material

Low Oxygen, Nitrogen Rich, Cold Atmosphere will be found Closer to the floor

oxygen monitors must be checked on a regular basis

Question 8

Chilling Injury

Answer 8

cryoprotectant agent(CPA) toxocity
-thermal shocke may cause miotic spindle dis assembly
-changing oocyte membane lipid composition
can be avoide by increasing cooling rate and using of anti freezing proteins

Question 9

what is false, regards vitrification:

a. vitrification involves placing embryos briefly in a solution to dehydrate them before they are rapidly frozen into a glass-like state
b. cryoprotective agents (CPAs) are used to eliminate ice formation when cooling organs to cryogenic temperatures; these cryoprotectants are sometime called as antifreeze
c. without a cryoprotectant, these crystals cause cell death
d. sucrose is expected to function as extracellular cryoprotectant to protect cell membranes from the effects of cold shock
e. propanediol is an efficient substitute for dimethyl sulfoxide in the vitrification of human oocytes and cleavage stage embryos
f. compare to other cryoprotectant glycerol is less toxic in high concentration and makes difficult to form ice crystals
g. chemicals called cryoprotectants are used during this process to prevent ice crystals forming, which could damage the embryos
h. embryos and oocytes could be cryopreserved without ice formation if there were no limit to the amount of CPA that could be used, but toxicity of CPAs limits the amount that can be used
i. there are so many cryoprotectants but glycerol, propylene glycol, dimethyl sulfoxide (DMSO) are most commonly used
j. DMSO NOT uses to prevent intracellular and extracellular crystals from forming in cells during the freezing process

Answer 9

j)