CRISPR Technology and Gene Therapy Flashcards
The stage of CRISPR immunity in bacteria in which the Cas complex integrates small pieces of viral DNA into the CRISPR array of the host cell
Adaptation (Acquisition)
The stage of CRISPR immunity in bacteria in which the CRISPR array is transcribed into a long pre-crRNA that is processed by other Cas proteins. crRNA then partially base pairs with tracrRNA and joins the Cas protein to form the effector complex
Expression
The stage of CRISPR immunity in bacteria in which complementary base-pairing occurs between crRNAs and viral DNA and Cas protein cleaves the viral DNA
Interference
The sequence that must be present in the target site for interference to occur
Protospacer Adjacent Motif (PAM) : 5’-NGG-3’
In engineered CRISPR Cas9 systems, the part that replaces the tracrRNA+crRNA
Single Guide RNA (sgRNA)
The part of the sgRNA that is target specific, 20nt
crRNA (Spacer Sequence)
The part of the sgRNA that is universal, 76 nt
tracrRNA
The part of the target DNA that is the DNA version of the spacer sequence
Protospacer
The part of the target DNA that is on the 3’ end of the protospacer
PAM sequence (NGG)
One of the mechanisms for the repair of double-strand breaks, error prone DNA repair pathway that created indels at random
Nonhomologous End-Joining (NHEJ)
One of the mechanisms for the repair of double-strand breaks, a DNA repair pathway that uses another DNA molecule to replace damaged DNA by homologous recombination
Homology-Directed Repair (HDR)
