CRISPR Flashcards
1
Q
sgRNA
A
a long sequence of nucleotide which is complementary to target gene
2
Q
PAM sequence
A
A sequence which allows the unwinding of DNA for sg RNA to check if complementary
distinguish non-self from self
3
Q
Cas9
A
protein which cuts DNA at a specific site (upstream of PAM site)
4
Q
Purpose of CRISPR in bacteria
A
Cas 1 and Cas 2 acquisition protiens collect viral DNA and store in spacer in CRISPR array. On second exposure, the sgRNA is sequenced from transcribing the spacer and attached to Cas9, sgRNA guides Cas9 to target sequence and deactivates virus by cutting the gene
5
Q
Side effects
A
- off-target effects
- immune response to treatment
6
Q
Advantages
A
- cost effective
- precise
- quick
