CPACs Flashcards
What is the enzyme used to break down milk protein
casein is broken down by trypsin
What do we put in colorometer
Cuvettes
How to cut cross section of plant stem
Put on white tile with some water to reduce friction. razor to cut
How to remove excess water/stain from slide
Touching edges with absorbent paper
What do we use to stain a stem cross section
Toluidine Blue
What does Toluidine Blue stain
Lignin and tannins green
Pectins Pink
Nucleic acid Purple
Why is hydrochloric acid used in observing root tip mitotic index
To break down the pectins holding cell together
Method to observe mitosis in root tip
- place garlic clove in conc HCL for 5 mins in hot water bath (55 degrees)
- Take out and rinse thoroughly
- Cut several root tips off
- stain with acetic orcein in water bath for five mins to increase staining
- Add a drop of water and macerate to spread out cells
- count mitotic index
What does acetic orcein stain and do
Chromosones dark red and fixes the cells in place
why do we add filter paper to each Petri dish when investigating pollen tube growth
To act as a humid chamber so the pollen slides do not dry out
How to get the pollen into the sucrose concentration
Rub the point of a mounted needle over the anthers into each solution
What filter do we use for beetroot colorimetery
Blue/green
What is plasmolysis
When the cell membrane separates from the wall
What do we use to measure water uptake by a plant shoot
Bubble potometer
How to set up potometer
Fill capillary tube and rubber connector with water. UNDER WATER put stem of shoot into rubber connector. Smear petroleum jelly around the shoot and rubber to make sure airtight. Blott the leaves to make them dry.
How to set up respirometer
Soda lime to absorb CO2 , gauze and then organisms. Attach to tube and place coloured bubble at end
How to prevent light from affecting some pondweed in a beaker
Cover with aluminium foil
What to add to water when measuring rate of photosynthesis underwater
Sodium hydrogen carbonate. Supplies hydrogen carbonate ions in water as a carbon source
What to crush leaves in to extract pigments for chromatography
Propanone
