Courtship Flashcards
What type of mutation is fruitless1?
An inversion
What is a non-autonomous P- element?
repeats but not coding for transposase
What elements do you need for P-element mutagenesis?
non-autonomous P-element and helper that codes for transposase
How do you discover where the fruitless mutation is with P-element mutagenesis
P element fails to complement mution , you see sterile male
How do you visualise where fruitless is?
In situ hibridization agains the P-element
What gene controls sexual determination in fruit flies?
The transformer gene
What is aggressive behaviour in females?
Headbutt
What is aggressive behaviour in males?
Lunges
Why is the fruitless gene longer in females than in males?
Due to alternative splicing
What Is TRA?
An RNA binding protein only expressed in females
What is the function of TRA?
To force the splicing to occur after the stop codon
HOw do you check Tra’s function?
With RT PCR using primers for females and males
HOw do you check Tra’s function?
With RT PCR using primers for females and males
What technique to use for checking expression of transcription factor in males?
immunohistofluorescence
What organs are necessary for cuticular hydrocarbon display?
Oenocytes
