Core Practical's: 18, 10, 11, 4, 9 and 16

Question 1

Core practical 4: Aim

Answer 1

Investigate the effect of enzyme and substrate concentrations on the initial rates of reactions.

Question 2

Core practical 4: Change

Answer 2

Concentration of enzyme

Question 3

Core practical 4: Organisms

Answer 3

Type of enzymes the same

Question 4

Core practical 4: Reliability

Answer 4

Repeat 4 times, calculate mean, discard anomalies

Question 5

4: Measure

Answer 5

Vol. of gas produced. In seconds. RATE - divide the volume by the number of seconds

Question 6

4: Same

Answer 6

Volume of distilled water, volume of enzyme, volume of substrate, concentration of substrate, PH, temperature

Question 7

4: Procedure

Answer 7

Wear eye protection, lab coats and disposable gloves. All enzymes are potential allergens and skin contact should be avoided. Any spillages onto the skin should be washed off immediately. Asthma sufferers may be particularly sensitive. Hydrogen peroxide is corrosive, therefore avoid contact with eyes, skin and clothing - any spillages onto the skin should be washed off immediately. Use the knife with care, cutting on a firm and secure surface.