Control of Gene Expression Flashcards
what are recognition sequences?
recognition sites for DNA binding proteins
can be close or far away from gene
what are gene regulatory proteins?
transcription factors that will bind and activate genes
associate w/ the major groove of DNA structure
gene regulatory proteins and their binding locations
protein surface is completely complimentary to surface of DNA binding region
contact has 4 possible configurations of base pairs
w/ 10-20 interactions
parts of gene regulatory proteins
DNA binding module
activation module
dimerization module
regulatory module
what modules are always present vs. could be present on gene regulatory proteins?
always – DNA binding, activation modules
might – dimerization, regulatory modules
dimerization module
could be present
forms dimers w/ other protein subunits
regulatory module
could be present
regulate the transcription factor
evidence for transcription factors being modular
using experiments where we cut out portions of the gene regulatory proteins we discovered that they are modular
aka certain regions do a specific thing
what are the 4 different structural motifs?
helix turn helix
zinc finger motif
leucine zipper
helix loop helix
what is the most common structural motif?
helix turn helix
2 alpha helices connected by a short chain of amino acids
helix turn helix
helix turn helix
turned at fixed angle
-the longer helix has the recognition and dna binding modules
-dna side chains recognize dna binding site
zinc finger motif
- -a zn atom located at the base of a finger-like structure
- -zinc finger domains found in clusters
- -have multiple contact points w/ dna = stabilization
2 alpha helical strands that contact DNA like a clothespin
leucine zipper motif
leucine zipper
- -activation and dimerization modules overlap
- -forms hydrophobics interactions between 2 helices = zipper
- -leucine every 7 amino acids
helix loop helix
a short alpha helix chain connected by a loop to a second chain
- -existing as a homo or heterodimer
- -3 modules: dna binding, dimer, activation
3 ways to identify transcription factors
EMSA
affinity chromatography
CHIP
electrophoretic mobility shift assay
EMSA
a gel mobility shift assay that detects sequence specific DNA binding proteins using a radioactive DNA fragment
after running a electrophoretic gel – the protein w/ smallest protein moves farthest – backpack example
affinity chromatography
to ID DNA binding proteins = purification
- ID a dna binding protein
- isolate
- use only 1 promoter recognition sequence
chromatin immuno-precipitation
CHIP
used when you don’t know what the regulatory protein binds to
–allowing you to ID sites in genome that a known protein binds to
CHIP use
used in living cells
PCR product at end so you can ID DNA sequence
how does histone acetylation effect binding to DNA
makes it easier to remove histones
thus easier access to DNA
activator and repressor protein competition
their binding sites might overlap and they can compete for binding
activator and repressor proteins both bind DNA
repressor can then bind to the activator to inhibit transcription = masking
or
repressor can bind to other DNA to block assembly of transcription machinery
what other factors can a repressor attract?
- -chromatin remodeling complexes
- -histone deacetylase
- -histone methyl transferase
chromatin remodeling complex recruitment
repressor can recruit these to fold up DNA and make promoter site unavailable