Contractile Proteins Flashcards
which type of actin has polarity: globular or filamentous?
filamentous
actin bundles
cross-linked actin into closely packed parallel arrays
actin networks
loosely cross-linked actin into orthogonal arrays that form 3D meshworks with gel-like properties
- more flexible
actin-bundling proteins
small, ridged proteins that force the filaments to align closely with each other
- cross-links actin
- determines the nature of association of filaments
fimbrin
actin-bundling protein; binds to actin filaments as a monomer; holds two parallel filaments closely together
- ex: microvilli
contractile bundles of actin
loosely bundled actin
- ex: contractile ring used in mitosis
alpha-actinin
actin bundling protein; allows motor protein (myosin) to interact during contraction; binds as a dimer; filaments of actin are separated by a greater distance which allows myosin to interact during contraction
filamin
actin bundling protein; binds actin as a dimer; can create 3D meshwork
spectrin
actin binding protein in RBCs; forms actin network that forms a cortical cytoskeleton; this network interacts with membrane proteins via interactions with ankyrin, protein 4.1
hereditary spherocytosis
decrease flexibility and stability of RBCs; caused by mutations in the cortical cytoskeleton proteins in RBCs (spectrin, ankyrin, 4.1)
- Sx: jaundice, anemia, splenomegaly
pseudopodia
type of actin projection that is responsible for phagocytosis
lamellipodia
broad, sheet like extensions of actin at the leading edge of a moving cell
filopodia
thin projections of plasma membrane supported by actin bundles; formation and retraction of filopodia is based on regulated assembly and disassembly of actin filaments
what driving force allows myosin to move along actin filaments
ATP hydrolysis
head domain of myosin
- two parts
contains actin binding and ATP binding sites; ATPase activity