Comprehension - L10-L11

Question 1

What do most proteins produced at the RER become?

Answer 1

glycosylated glycoproteins

Question 2

What is N-linked glycosylation?

Answer 2

• Linkage to asparagine
• Is initiated in the RER

Question 3

What is O-linked glycosylation?

Answer 3

Linkage to serine and threonine
occurs in the Golgi complex

Question 4

How do things exit the ER?

Answer 4

1. Membrane vesicles bud from the ER
2. Transport vesicles fuse with one another to form VTCs in the ERGIC

Question 5

What happens in the Trans Golgi Network?

Answer 5

Sorting station where proteins are sorted into diff types of vesicles

Question 6

What happens in the Cis Golgi network?

Answer 6

Sorting station that distinguishes between proteins that need to be returned tot he ER and those that should proceed through Golgi

Question 7

What determines the ordered that sugars are incorporated?

Answer 7

The location of specific glycosyltransferases

Question 8

What model explains movement through the Golgi?

Answer 8

Cisternal maturation model

Question 9

What do Clathrin-coated vesicles do?

Answer 9

Move materials from the TGN to: endosomes, lysosomes, and plant vacuoles
(endocytosis)

Question 10

What do COP1-coated vesicles do?

Answer 10

Move cargo backwards
- From ERGIC/Golgi to ER
- From trans to cis Golgi

Question 11

What do COP2-coated vesicles do?

Answer 11

Move cargo forward from the ER to Golgi complex

Question 12

Which protein acts as a gatekeeper in the ER quality control system by catalyzing monoglucosylation onto incompletely folded glycoproteins, allowing them to recognized by ER chaperons.

Answer 12

UGGT

Question 13

What are two main functions of the COP2 coat proteins?

Answer 13

1. Bend the membrane
2. Select and concentrate the proteins to be transported

Question 14

What is the membrane bending G protein called in COP1 coated vesicles?

Answer 14

Arf1

Question 15

How do vesicles move towards the specific target compartment?

Answer 15

Movement is mediated by microtubules and motor proteins

Question 16

What do Rabs do?

Answer 16

Recruit specific tethering proteins and interact with specific motor proteins

Question 17

Where are t-SNAREs located

Answer 17

Target membrane

Question 18

Where are v-SNAREs located?

Answer 18

Put into vesicles during budding

Question 19

What do lysosomes contain?

Answer 19

Lysosomes contain at least 50 hydrolytic enzymes

Question 20

How is the pH of the lysosome maintained?

Answer 20

By a proton pump
V-Type H+ATPase