Component Processing Wk5

Question 1

Components of blood

Answer 1

Red cells (erythrocytes)

White cells (leucocytes)

Platelets (thrombocytes)

Plasma (containing clotting factors and antibodies)

Question 2

Blood component processing

Question 3

Why process into compounds

Answer 3

Enables NHSBT make effective use of donations
Allows leucodepletion of products to <5x10^6 / L WBC
Enriches constituents
Better storage of individual components
Specific products used for specific problems

Question 4

Manufacture of red cells and FFP

Answer 4

60% of Donation bled into whole blood filter pack
Pack 1 - CPD anticoagulant
Pack2+3- empty, 1 white cell filter
Pack 4- SAGM

Question 5

BD filtered into empty pack= leucocyte depleted whole blood
Donation centrifuged hard at 4C
Placed into opitpress blood seperator

Answer 5

Optipress squeezes pack = remove plasma to empty pack= FFP <8 hours men. bin>8hours leaving red cells
SAGM drained to pack containing red cells

Plasma blast frozen to -40C = FFP. If <8 hours since donation and male
Red cells stored at 4C

Question 6

Manufacture of red cells + platelets

Answer 6

Donations bled into bottom + top bags BAT
40% donations bled
4 packs, top of donor, 2 packs joined together “train” form from bottom of one - contains SAGM

Question 7

Donation centrifuged softly. = coat layer containing white cells + platelets
Placed in optipress blood separator
Squeezes pack to remove plasma into empty pack = discarded

Answer 7

+ remove red cells via leucocyte filter into empty pack, SAGM drained in = red cells stored at 4C
Left with buffy coat in original pack (platelets + white cells)

Question 8

Making platelets from buffy coats

Answer 8

4 joined together = train = pooled with 200ml male plasma of same group into bag
Centrifuge gently = platelet rich plasma (PRP)
Optipress squeezes PRP via leucocyte filter into final pack.
Stored at RT with agitation

Question 9

Apheresis platelets

Answer 9

30% platelets produced from apheresis donors

Question 10

Manufacture of cryoprecipitate

Answer 10

Cryo = plasma product
Contains factors VIII + fibrinogen
Male frozen plasma thawed at 4C up to 18 hours
Cryoprecipitate formed
Excess plasma removed, remaining cryo redissolved
Pool of 5 donations

Question 11

Last step

Answer 11

All components weighed + stored in quarantine
When donation testing complete products labelled with blood group, expiry date + phenotype info
Failures discarded, donor follow up
Issued to hospital

Question 12

Storage + exiary dates