COMPLETE BLOOD COUNT Flashcards
What stain is used for staining PBS
Wright-Giemsa Stain
Troubleshooting;
RBCs appear gray
WBCs are too dark
Eosinophil granules are gray, not orange
Stains are too alkaline
Inadequate rinsing
Prolonged staining
Heparinized specimen
Troubleshooting;
RBCs are too pale
RBCs are too red
WBCs are barely visible
Stains are too acidic
Under-stained
Overrinsing
Reference interval of WBC
4.3-10.8 x 10^9/L
Reference interval of RBC for male
4.20 - 6.00 x10^6/L
Reference interval of RBC for female
3.80 - 5.20 x 10^6/L
Reference interval of Hemoglobin for male
13.5 - 18.0 g/dL
Reference interval of Hemoglobin for female
12 - 15 g/dL
Reference interval of Hematocrit for male
40 - 54%
Reference interval of Hematocrit for female
35 - 49%
Reference interval of MCV
80-100 fL
Reference interval of MCH
26 - 32 pg
Reference interval of MCHC
32 - 36 g/dL
Reference interval of RDW
11.5 - 14.5%
Reference interval of PLT
150-450 x 10^3/uL
Reference interval of RETIC
20-115 x 10^3/uL
0.5-2.5%
Relative Differential Count of Neutrophil
48-70%
Relative Differential Count of Lymphocytes
18 - 42%
Relative Differential Count of Monocyte
1 - 10%
Relative Differential Count of Eosinophil
1 - 4%
Relative Differential Count of Basophil
0 - 2%
Method for hemoglobin determination
Cyanmethemoglobin
In hemoglobin determination blood is diluted in
Drabkin solution
Hematocrit of Newborn
48 - 68%
Hematocrit of Infant
38 - 48%
Bore size of capillary tube
2mm
Length size of capillary tube
75mm
Microcytic and Hypochromic
TAILS
Thalassemia
Anemia of chronic disease
Iron deficiency anemia
Lead poisoning
Sideroblastic anemia
Normocytic and Normochromic
AHAS
Aplastic anemia
Hemolytic
Acute blood loss
Sickle cell anemia
Macrocytic and Normochromic
Pernicious anemia
Megaloblastic anemia