collection of specimens

Question 1

how many specimen should you look at?

Answer 1

3-5 specimens at different intervals (different days)

Question 2

how much of specimen would you miss if only collect 1 specimen?

Answer 2

30%

Question 3

even if you find 1 specimen in the stool

Answer 3

keeping looking for more

Question 4

when should you not collect from patients

Answer 4

after barium enemas– will mask parasites

Question 5

specimens that can be collected

Answer 5

urine, blood, stool, other body fluids

Question 6

what is main specimen

Answer 6

stool- find when stool is softer/liquid

Question 7

why place stool in a preservative

Answer 7

keeps morphology of stool and preserve without further maturation or deterioration

Question 8

what kind of stool is cysts found in

Answer 8

formed stool or soft

Question 9

what kind of stool is trophs find in

Answer 9

loose or watery

Question 10

what is gold standard preservatives

Answer 10

SAF- sodium acetate-acetic acid formalin

Question 11

what is SAF good for

Answer 11

used for immunoassay testing

Question 12

disadvantages of PVA

Answer 12

not good with wet mounts- cloudy and can’t be used with immunoassay

Question 13

wet mounts can be done when

Answer 13

concentrated or non concentrated specimen

Question 14

concentration technique done on

Answer 14

urine or stool

concentrate amount of parasites and remove debris

fresh/preserved stools

Question 15

biggest drawback of concentration technique

Answer 15

Trophozoite stage with protozoans may be destroyed in concentration

Question 16

floatation method

Answer 16

eggs and things float to top using zinc sulfate

Question 17

disadvantages of flotation method

Answer 17

Some dense eggs are heavy and may not float to the top = missed
Operculated eggs- opening in egg ; will fill with fluid and sink

Question 18

Sedimentation method

Answer 18

get concentrated by centrifuging

uses ethyl acetate

most COMMON

Question 19

common permanent stain for slide making

Answer 19

Trichrome stain – gives blue/greenish background
- Enhances morphology
-Stained after concentration
KNOWN as Wheatley’s modification

Question 20

other kind of permanent slide making stain

Answer 20

Iron hematoxylin stain- grayish color
- After specimen has been concentrated = stained
-Considered gold standard

Question 21

what helps size parasite

Answer 21

micrometer

Question 22

what is breakdown of eosinophils (pink,sharp looking)

Answer 22

charcot- leyden crystals