CMB1003/L04 Cultivating Bacteria Flashcards
Give 2 methods of staining bacteria.
Gram stain
Chemical stain
What 2 groups can bacterial nutrients be divided into?
Macronutrients
Micronutrients
What percentages of C, O, N and P do bacteria need?
C - 50%
O - 17%
N - 13%
P - 2.5%
How can carbon and oxygen be provided to bacteria?
Sugars, amino acids
H2O or O2
How can nitrogen be provided to bacteria?
NH4Cl, (NH4)2SO4
How can phosphorus be provided to bacteria?
KH2PO4, Na2PO4
Give the 2 types of media.
Chemically defined media
Undefined complex media
What is a defined media?
Contains specific nutrients for a given bacterial species under certain conditions
In which 2 forms can bacteria be fed?
Liquid broth of solid agar
What can be measured from a batch culture?
Population density
Population number
What can be calculated from a batch culture?
Growth rate
Generation time
Describe a colony forming unit (CFU). (2)
Assumes that a colony grows from a single cell
Extrapolated to give cell numbers in original culture
What is the equation for generation time?
Generation time = time / number of generations
Define extremophile. (2)
An organism that lives under harsh conditions
Would kill most other organisms
Where is optimum pH based?
On extracellular environment
Cytoplasmic is around pH 7
