CLT 252 EXTRA CREDIT

Question 1

The end product in the colloidal iron method is Prussian blue.
- True
- False

Answer 1

• True

Question 2

Which of the following stains is used exclusively for liver histopathology?
a. Gordon and Sweets Reticulin
b. Masson Trichrome
c. H & E
d. Kinyoun acid-fast method

Answer 2

a. Gordon and Sweets Reticulin

Question 3

Absolute alcohol must be used as the fixative for the:
a. Grimelius technique.
b. Gomori methenamine silver method.
c. Churukian-Schenk process.
d. Fontana-Masson

Answer 3

b. Gomori methenamine silver method

Question 4

The purpose of Cresyl Echt Violet Method I is to:
a. To detect the presence of acid-fast bacteria.
b. Demonstrate neutral mucosubstances.
c. Show nerve fibers in cross-section.
d. Identify loss of Nissl substance in tissue

Answer 4

d. Identify loss of Nissl substance in tissue

Question 5

When viewed with a light microscope, a paraffin section from tissue know to contain urates crystals in a gouty tophus fails to reveal any staining with methenamine silver. The presence of urate crystals could be verified by examining the tissue with which of the following?
a. polarizing
b. dark field
c. phase contrast
d. fluorescence

Answer 5

a. polarizing

Question 6

The purpose of Best Carmine is to:
a. Demonstration of Glial Cells
b. determination of the type of fungi
c. Demonstration of glycogen
d. Demonstration of bile pigments

Answer 6

c. Demonstration of glycogen

Question 7

Formalin ammonium bromide must be used for this technique :
a. Cajal
b. Holzer
c. Bielschowsky-PAS stain
d. Holmes silver-nitrate

Answer 7

a. Cajal

Question 8

Which of the following is false in regard to the Gram stain?
a. The Gram stain is used to classify microorganisms in the laboratory.
b. Gram positive organisms appear purple or deep blue because of teichoic acid in their cell wall
c. Gram negative bacteria appear pink or red due to lipopolysaccharides in their cell walls.
d. The Gram stain is used to detect the presence of acid-fast bacteria.

Answer 8

d. The Gram stain is used to detect the presence of acid-fast bacteria.

Question 9

Which of the following is false about Cornflaking?
a. Sections should always be air-dried before mounting.
b. Can be fixed by using Xylene to remove the coverslip and mounting media. Then rehydrate and clear by placing in water.
c. Avoid this situation by not letting the slide dry before mounting and coverslipping.
d. Can consist of black areas resembling fungi.

Answer 9

a. Sections should always be air-dried before mounting.

Question 10

During H & E staining, white spots appearing in the paraffin is most likely caused by:
a. too much time in Xylene.
b. mineral precipitation
c. over-drying.
d. Water left in the tissue

Answer 10

d. Water left in the tissue