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CLT 252 > CLT 252 EXAM 2 > Flashcards

CLT 252 EXAM 2 Flashcards

1
Q

Which of the following must be added to link hematoxylin to tissue DNA?
a. acid
b. oxidizer
c. stabilizer
d. mordant

A

d. mordant

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2
Q

A good control for Mayer mucicarmine stain is:
a. kidney
b. appendix
c. spleen
d. liver

A

b. appendix

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3
Q

The Schiff reaction demonstrates:
a. hyaluronic acid
b. aldehydes
c. basophilic compounds
d. amphoteric tissue components

A

b. aldehydes

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4
Q

The most important step in regressive hematoxylin staining is:
a. post mordanting in picric acid
b. use of hematoxylin containing glycerin
c. differentiation in acid-alcohol
d. washing in water after the hematoxylin

A

c. differentiation in acid-alcohol

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5
Q

Amyloid can be demonstrated with:
a. thioflavin T
b. Mayer mucicarmine
c. cresyl echt violet
d. alcian blue

A

a. thioflavin T

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6
Q

Black precipitate is seen on sections stained with the Gomori method for reticulin. This is most likely the result of:
a. over-rinsing the slide prior to the formaldehyde
b. leaving the slides too long in gold chloride
c. using glassware that was not chemically cleaned
d. inadequately rinsing the slide after the nuclear fast red

A

c. using glassware that was not chemically cleaned

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7
Q

The impregnating solution used in the Bodian method is:
a. iron hematoxylin
b. silver protein
c. mercuric chloride
d. aniline blue

A

b. silver protein

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8
Q

The crystal violet stain for amyloid is a/an:
a. polarization technique
b. physical reaction
c. impregnation method
d. polychromatic stain

A

d. polychromatic stain

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9
Q

Which of the following stains could be used to demonstrate cirrhosis of the liver?
a. Weil
b. Holzer
c. Masson trichrome
d. Bielschowsky

A

c. Masson trichrome

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10
Q

Fat is chemically fixed and maintained in tissue by:
a. osmium tetroxide
b. mercuric chloride
c. calcium formol
d. Carnoy solution

A

a. osmium tetroxide

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11
Q

Harris Hematoxylin is used on tissues to stain:
a. fat
b. glycogen
c. nuclei
d. cytoplasm

A

c. nuclei

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12
Q

The oil red O stain might be used to demonstrate:
a. rhabdomyosarcomas
b. leiomyosarcomas
c. liposarcomas
d. adenocarcinomas

A

c. liposarcomas

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13
Q

The alcian blue stain performed at pH 1.0:
a. all acid mucopolysaccharides
b. carboxylated acid mucopolysaccharides
c. sulfated acid mucopolysaccharides
d. neutral mucopolysaccharides

A

c. sulfated acid mucopolysaccharides

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14
Q

Glycogen is best demonstrated by the use of:
a. Crystal violet colloidal iron
b. Mayer mucicarmine
c. PAS with and without diastase
d. alcian blue with and without hyaluronidase

A

c. PAS with and without diastase

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15
Q

Which type of metal salt serves as the mordant in Weigert hematoxylin?
a. iron
b. aluminum
c. mercury
d. tungsten

A

a. iron

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16
Q

A good control for glycogen is:
a. kidney
b. appendix
c. lung
d. liver

A

d. liver

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17
Q

Of the following, which is the best fixative for glycogen?
a. Zenker
b. Orth
c. acetic acid
d. absolute alcohol

A

d. absolute alcohol

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18
Q

Which of the following is not true of adipose cells:
a. Granules stain metachromatically
b. Demonstrated by oil red O
c. Are connective tissue cells
d. Demonstration requires frozen sections

A

a. Granules stain metachromatically

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19
Q

Plasma cells can be demonstrated with:
a. methyl green-pyronin
b. Verhoeff-van Gieson
c. toluidine blue
d. PAS

A

a. methyl green-pyronin

20
Q

Which of the following is a breakdown product of hemoglobin?
a. melanin
b. ceroid
c. bile
d. lipofuscin

A

c. bile

21
Q

Granular deposition of the silver is seen on the control stained with Gordon and Sweets reticulin method. One possible explanation is that:
a. this is the normal result of Gordon and Sweets method
b. the reagents may have been old
c. the sections were not properly toned
d. the sections are too thick

A

b. the reagents may have been old

22
Q

DNA can be demonstrated with:
a. eosin
b. pyronin
c. the Feulgen reaction
d. fast green

A

c. the Feulgen reaction

23
Q

Which of the following methods is Fluorescent microscopy used?
a. Holzer
b. Bielschowsky
c. Cajal
d. thioflavin S

A

d. thioflavin S

24
Q

The connective tissue failed to stain yellow with the Movat pentachrome stain. One possible cause is that the :
a. metanil yellow is too dilute
b. safran solution contains water, it must be tightly capped to prevent hydration and evaporation.
c. Phosphotungstic acid was removed with acetified water
d. tissue was fixed in an acetic-formalin mercury solution

A

b. safran solution contains water, it must be tightly capped to prevent hydration and evaporation.

25
Q

The active staining chemical in ripened hematoxylin is:
a. hematein
b. Harris solution
c. hematoxylin
d. hemosiderin

A

a. hematein

26
Q

Which of the following is not true of the Gram stain?
a. will stain bacteria
b. uses crystal violet
c. preferred for the demonstration of Legionella pneumophila
d. Differentiates between different types of bacteria

A

c. preferred for the demonstration of Legionella pneumophila

27
Q

Nissl substance is composed of:
a. rough endoplasmic reticulum
b. neurofilaments
c. glial fibers
d. microtubules

A

a. rough endoplasmic reticulum

28
Q

Colloidal iron is used for the demonstration of:
a. amyloid
b. glycogen
c. acid mucopolysaccharides
d. neutral mucopolysaccharides

A

c. acid mucopolysaccharides

29
Q

The control for the Hall stain must contain;
a. hemosiderin
b. hemoglobin
c. urate crystals
d. bile

A

d. bile

30
Q

Which of the following is true of the Fite method?
a. used for the demonstration of mycobacteria
b. depends on the formation of aldehydes
c. is an argyrophilic technique
d. preferred for the demonstration of Legionella pneumophila

A

a. used for the demonstration of mycobacteria

31
Q

A stain that may be used to demonstrate calcium is the:
a. Gomori chromium hematoxylin
b. Fontana-Masson
c. von Kossa silver nitrate
d. Schmorl method

A

c. von Kossa silver nitrate

32
Q

Which of the following is responsible for the color of white matter?
a. astrocytes
b. microglia
c. Nissl substance
d. myelin

A

d. myelin

33
Q

The auramine-rhodamine technique will demonstrate:
a. Staphylococcus aureus
b. Mycobacterium tuberculosis
c. Candida albicans
d. Clostridium tetani

A

b. Mycobacterium tuberculosis

34
Q

The best stain for the demonstration of Mycobacterium leprae is the:
a. Fite
b. PAS
c. Kinyoun
d. Gram

A

a. Fite

35
Q

Sections to be stained for myelin should be cut at:
a. 4-5 µm
b. 6-8 µm
c. 10-15 µm
d. 20-30µm

A

c. 10-15 µm

36
Q

Which of the following is a method for the demonstration of Fungi?
a. Ziehl-Neelsen
b. Warthin-Starry
c. Giemsa
d. PAS

A

d. PAS

37
Q

The basic structure of filamentous fungi is the:
a. hypha
b. bud
c. endospore
d. spherule

A

a. hypha

38
Q

Which of the following stains requires mounting with aqueous mounting media?
a. Fite
b. oil red O
c. Grocott
d. H & E

A

b. oil red O

39
Q

Synthetic resins are preferred over natural resins because the synthetic resins:
a. dry more slowly, preventing cracking of resin
b. indicate the age of preparations by turning yellow
c. preserve the intensity of the stain with time
d. have a slight acidity when dissolved in xylene

A

c. preserve the intensity of the stain with time

40
Q

Which of the following is not true for the Kinyoun stain?
a. Is a fluorescence method
b. Results are easily contaminated by tap water
c. The staining solution contains phenol
d. Demonstrates mycobacteria

A

a. Is a fluorescence method

41
Q

A medically important protozoan is:
a. Staphylococcus aureus
b. Giardia lambia
c. Candida albicans
d. Clostridium tetani

A

b. Giardia lambia

42
Q

Nissl substance is present in:
a. astrocytes
b. neurons
c. Schwann cells
d. microglia

A

b. neurons

43
Q

A tissue frequently used as a control for the Fontana-Masson stain is:
a. skin
b. lymph node
c. liver
d. spleen

A

a. skin

44
Q

Which of the following is not true of the Luxol fast blue method?
a. Is a regressive method of staining
b. Differentiation is begun with an alkaline solution
c. demonstrates myelin in tissue
d. Demonstrates glial cells

A

d. Demonstrates glial cells

45
Q

Artificial precipitates that are seen in the Grocott stain may be the result of using:
a. plastic forceps
b. gelatin in the water bath
c. glassware that was not chemically cleaned
d. excess time in the gold chloride solution

A

c. glassware that was not chemically cleaned

CLT 252 (4 decks)

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