Clostridia and Bacillus

Question 1

Where in the normal flora does your Clostridium live?

Answer 1

colon

Question 2

Listeria bacillus and Clostridium are both Gram positive Bacilli, but you can tell them apart by testing because

Answer 2

grow in different conditions
Listeria is Aerobic
Clostridium is Anaerobic

Question 3

What microbe do i really need to worry about because i’m always reheating rice?

Answer 3

bacillus cereus

Question 4

What else can C. perfringens cause besides watery diarrhea?

Answer 4

Gangrene (scary)

Question 5

what toxins are produced by C. perfringens?

Answer 5

alpha toxin
beta toxin
E toxin
I- toxin 
Enterotoxin

Question 6

What does alpha toxin do?

Answer 6

lethal necrotizing lecithinase

Question 7

what does beta toxin do?

Answer 7

causes intestinal stasis, mucosal loss and necrotic lesions can progress to necrotizing enteritis

Question 8

what does E toxin do??

Answer 8

causes increased vascular permeability of GI tract

Question 9

what does I-toxin do?

Answer 9

causes increased vascular permeability of GI tract

Question 10

what does Enterotoxin do?

Answer 10

alters membrane perm in small intestine leading to loss of fluid and ions superantigen.

Question 11

What does C. perfringes food poisoning do to you? how long does it last? How long does it incubate?

Answer 11

incubation: 8-24
gives you watery diarrhea, no fever, nausea or vomiting.
lasts 24-48

Question 12

How do you detect C. perfringens in lab diagnosis?

Answer 12

From contaminated food

or from patient

Question 13

How do you treat C perfringens?

Answer 13

antibiotics

Question 14

Bacillus anthracis causes a disease in humans, what is it, and is it gram positive or negative?

Answer 14

It is gram positive and it causes anthrax

Question 15

B cereus is a bacillus that causes symptoms in humans, what are they?

Answer 15

Gastroenteritis and serious ocular infections

Question 16

What 3 major soft tissue infections infections are associated with C perfringens?

Answer 16

Cellulitis with formation fo gas
Faciitis or suppurative myositis
Clostridial myonecrosis/ gas gangrene

Question 17

A large gram positive rod, strict anaerobe, produces round terminal spores, “drumstick” appearance, motile,found in GI tract comes from soil. Comes in thru skin breaks. what is it?

Answer 17

C. tetani

Question 18

What two toxins do C. tetani produce?

Answer 18

Tetanolysin

Tetanospasmin

Question 19

If a bacteria is a large, gram POSITIVE rod with SQUARE ends found in a chain, a facultative aneorobe that produces spores and is encapsulated and non motile, infects herbivores, can cause cutaneous symptoms (black ulcer) pulmonary symptoms (flu then death) and rarely GI symptoms what is it?

Answer 19

Bacillus Anthracis

Question 20

If a bacteria is a largre gram POSITIVE rod, a STRICT aneirobe that produces spores, exists in intestines of SOME healthy people, produces Toxin A and B and is often triggered by generalized antibiotics, causes diarrhea usually watery and Pseudomembranous colitis what is it?

Answer 20

Clostridium Difficile

Question 21

If a bacteria is a large Gram Positive rod, a strict anaerobe (fastidious) that produces spores, found in soil but usually infects people from improperly canned food, causes nerve damage related to acetylcholie causes FLACCID paralysis, double vision weakness and dizziness, can be transmittedd to infants then what is it?

Answer 21

Clostridium Botulinum