Clin Lab Med Exam II Flashcards

1
Q

Epithelial cells

A

contaminated specimen

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2
Q

Oval fat bodies

A

Nephrotic syndrome

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3
Q

Hyaline cast

A

Normal, most common

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4
Q

RBC cast

A

Glomerulonephritis

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5
Q

WBC cast

A

Acute pyelonephritis

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6
Q

Renal tubule cast

A

Acute tubular necrosis

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7
Q

Specific gravity (UA)

A

Acute kidney failure

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8
Q

Glucose (UA)

A

Diabetes

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9
Q

Ketones (UA)

A

Acidosis (DKA)

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10
Q

Protein (UA)

A

Kidney dz

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11
Q

Moderately increase Albumin (UA)

A

Early Kidney dz

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12
Q

Bilirubin/ Urobilinogen (UA)

A

Liver dz
Hemolysis
Biliary obstruction

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13
Q

Uric acid crystals

A

Hyperuricemia

Gout

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14
Q

Struvite crystals

A

Alkaline urine

Infection by urease producing bacteria

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15
Q

Hypokalemia

Low K

A
Causes:
Vomiting/gastric suction
Thiazide//loop diuretics
Alkalosis
Insulin
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16
Q

Kyperkalemia

A

Causes:
ACE-I, ARB
Aldosterone antagonist
K-sparing diuretics

Acidosis

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17
Q

Tx for Hyperkalemia

A

Insulin/Ca/Bicarb administration
Loop/Thiazide diuretics
Dialysis

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18
Q

Normal range of sodium

A

135-145

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19
Q

Solutes that determine ECF osmolality

A

Sodium
Glucose
Urea

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20
Q

Sx occur if osmolality is out of whack by this much

A

<265

>320

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21
Q

Substances that are active but not included in calculated osmolality (lead to Osmolol Gap)

A
Mannitol
Other proteins
Ethanol
Methanol
Ethylene glycol (antifreeze)
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22
Q

Tonicity

A

ability of all solutes to make Osmotic Driving Force that causes water mov from one compartment ot another

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23
Q

Examples of solutes unable to cross from ECF to ICF that influence tonicity

A

Sodium
Glucose
Mannitol

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24
Q

What is a substance that crosses freely and therefore does NOT contribute to tonicity?

A

Urea

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25
Major determinant of the size of ECF volume
(Na) Sodium
26
Increase Na in ECF
Hypervolemia
27
Serum [Na+]
refers to amt of water relative to sodium in ECF | not the total body Na+ amt
28
Abnormal Serum [Na+] means
disorder of water regulation | not necessarily a messed up sodium level
29
Abnormal ECF volume
marker of abnormal (Na) sodium control
30
Causes of Hypovolemia
``` GI loss Renal loss (diuretics, Diabetes Insipidus) Skin loss (sweat, burn) Intestinal obstruction, pancreatitis, Rhabdo ```
31
Hypovolemia sx
``` Weakness Muscle cramps Decreased BP Postural hypotension Increased pulse ```
32
Causes of HYPERvolemia
``` Renal failure Nephrotic synd Primary Hyperaldosteronism Cushing's synd Liver dz Heart failure Pregnancy ```
33
3 main causes of HYPER volemia
Kidney failure Liver failure Heart dz
34
Signs of HYPERvolemia
``` Edema SOB Orthopnea (PND) JVD Hepatojugular reflux Crackles ```
35
Water retention influenced by:
Thirst | ADH
36
Salt retention influenced by:
RAAS ANP Catecholamines Renal fx (GFR, BF)
37
Aldosterone actions
Increase Na absorption | Kicks K out of body
38
Severe Hyponatremia (sodium so low, in danger zone)
below 125
39
Most common electrolyte abnormality in hospitalized pts
Low sodium
40
Hyponatremia is assoc/ with
Pulmonary dz | CNS disorder
41
Signs of low sodium
``` HA, dizzy N/v Lethargy Weakness Confusion Severe: Hypoventilation, Resp arrest, Seizure, Coma, Death ```
42
Pseudohyponatremia
Falsely low serum sodium, <135 BUT osmolality is normal
43
Pseudohyponatremia (falsely low sodium) occurs with:
Hyperlipidemia | Hyperprotein
44
Redistributive or Hyperosmolar Hyponatremia
Osmotically active particles in the ECF are concentrating stuf out there and drawing water out of the cell as a result, diluting the Na concentration outside of the cell
45
Common cause of Redist/Hyperosmolar Hyponatremia (low sodium)
Hyperglycemia
46
Hyperglycemia is often seen with
Hyponatremia | type: Hyperosmolar/Redistributive
47
Hypervolemic Hyponatremia
too much volume | too little Na
48
Cause of Hypervolemic Hyponatremia | too much fluid, too little sodium
Liver, Heart, or Kidney failure | Tx: diuretics, dialysis, fluid restrict
49
Hypovolemic Hyponatremia | low volume, low sodium
Check Urine Na
50
If urine Na >20 in Hypovolemic Hyponatremia
Cerebral salt wasting Renal tubular acidosis Diuretics (thiazide)
51
If urine Na <20 in Hypovolemic Hyponatremia
Vomiting/diarrhea | Third space loss (burn, pancreatitis)
52
Tx of Hypovolemic Hyponatremia (low everything)
Replace with fluids | Tx underlying cause
53
Euvolemic Hyponatremia
SIADH Psychogenic polydipsia Hypothyroid Adrenal insuff
54
Tx of Euvolemic Hyponatremia
Fluid restriction | Tx underling cause
55
SIADH too much ADH retaining water
TOO MUCH ADH activity Keeps water in but lets Na out Serum diluted Urine concentrated
56
Determine cause of SIADH
CT/MRI head (CNS disorder) CXR (lung tumor/inf) Review pt meds
57
SIADH dx criteria (5 things)
1. diluted plasma <275 2. concentrated urine >100 3. elevated urine Na >20 4. euvolemic 5. normal cortisol and thyroid levels
58
Dx Hyponatremia, First look at
Serum: Na, osmolality
59
Dx Hyponatremia, 2nd look at
Urine Na and osmolality
60
Dx Hyponatremia, 3rd look at
TSH, Serum Cortisol
61
When to admit a pt to hospital for Hyponatremia?
If sodium is below 125 OR symptomatic
62
Common use for tx of Chronic Hyponatremia
"Vaptans" and | Demeclocycline
63
Correcting Low Sodium
4-6 in first 24 hours definitely less than 8
64
How often to check sodium when you are correcting levels
every 2 hours
65
CPM- Central Pontine Myelinolysis
Aka osmotic demyelination synd Focal demyelination in the pons Irreversible!
66
Sx of CPM- Central Pontine Myelinolysis
Dysarthria, dysphagia, seizures, AMS, quadriparesis, hypotension 1-3 days after over-correction
67
Hypernatremia
too little water relative to salt | >145
68
Clinical features of Hypernatremia d/t
brain shrinkage
69
Clinical sx of Hypernatremia (if any, often asymptomatic)
``` Thirst AMS, weakness Neuromusc irritability Focal neuro deficits Seizure/coma ```
70
Causes of Hypernatremia
``` GI loss (elderly or infants w diarrhea) Sweating, fever Renal loss Diuretics Osmotic diuresis ```
71
Vast majority of Hypernatremia are d/t:
Water loss | GI, skin, renal
72
Body's normal response to Hypernatremia
Thirst and fluid intake
73
Diabetes Insipidus dilute urine concentrated blood
Losing a bunch of dilute water (collecting ducts are impermeable to water and cannot reabsorb it) Concentrated serum Na
74
Causes of DI
Central: impaired secretion of ADH tx: desmopressin Nephrogenic: lack of kidney response to normal amts of ADH
75
SIADH
too much ADH!
76
Diabetes Insipidus
not enough ADH or impaired response to ADH!
77
Tx of Nephrogenic DI | we want to get rid of Na
when kidneys aren't responding to normal amts of ADH - Thiazide diuretics (cause you to get rid of Na) - Amiloride - Chlorpropamide - NSAIDs
78
Tx of Hypernatremia
Stop water loss Replace water deficit Hospitalize (if severe)
79
Water deficit
Normal TBW- current TBW
80
3 parts to UA
Gross examination Dipstick Microscopic analysis
81
What can cause urine to be red or red-brown
Beets and Rhubarb
82
What can cause urine to be brown-black?
Biliary dz Alkaptonuria Malignant melanoma
83
Maple syrup urine dz and Phenylketonuria
Amino acid disorder
84
Normal pH for Urine
4.5-8.0
85
Urine is water containing dissolved:
Urea, Na, and Cl (some other stuff)
86
Normal Spec Gravity of Urine
1.003-1.035
87
Spec Grav reflects kidney's ability to
Concentrate and dilute the urine
88
In kidney dz, Spec Grav may be fixed at
1.01 | "Isothenuria"
89
Urine volume normal
500-2000 Olig: <500 Anuria: <100
90
False negatives for Glucose in urine
Absorbic acid | ASA
91
Ketones
product of incomplete fat metabolism, occur when carbs diminished
92
Ketones in urine
Acidosis | DKA, rapid weight loss, fasting, starvation, pregnant
93
Albumin
indication of Renal endothelial dysfx | Early sign of kidney dz
94
False positive Albuminuria test
Pyridium can cause false positive
95
Limit to Albuminuria test
Low levels: 30-300 usually not detectable with dipstick
96
Alb to Cr ratio used for screening for Kidney Damage in high risk pts
Diabetes HTN Cardiovascular dz
97
Persistently positive dipstick for protein should:
also have Albuminuria quantified
98
Blood in urine can mean 3 things:
RBC Hemoglobin Myoglobin
99
False negative to blood can be d/t
Ascorbic acid
100
High power field
WBC | RBC
101
Low power field
Casts
102
RBC
>3 in urine is abnormal
103
If sediment is red
Hematuria (RBC)
104
If supernatant is red, do a dipstick heme
if negative, red color is d/t something else
105
If supernatant is red, do a dipstick heme
if positive, centrifuge again and determine color Clear: myoglobin Red: hemoglobin
106
WBC lobed nuclei refractile cyto granules
>5 is abnormal 10-20 suspicious >20 probably UTI
107
Tubular and transitional epithelial cells
tubular degeneration if high numbers
108
Oval fat bodies
"Maltese cross" | Nephrotic syndrome
109
"Maltese cross"
seen by Polarized light microscopy | Nephrotic synd
110
Where are casts formed?
Distal tubule or Collecting duct
111
Red cell casts
Glomerulonephritis
112
White cell casts
Pyelonephritis
113
Renal tubular casts
Acute tubular NECROSIS
114
Cystine crystals "sisteine chapel genetically caused stones"
rare Genetic cause of kidney stones
115
Struvite
Urease producing bacterial infection
116
Calcium oxylate
kidney stones
117
crystals that cause Kidney stones
Cystine (rare genetic) | Calcium oxylate
118
Regulation of Renal K excretion occurs in
Distal nephron
119
Too much K | Hyperkalemia
Serum level >5
120
Causes of too much K | Hyperkalemia
Pseudo Inadequate kidney excretion Redistribution Excess administration
121
Aldosterone leads to low K levels
so anything that antagonises Aldosterone will ---> too much K (Hyperkalemia)
122
Causes of Hyperkalemia (too much K)
Aldosterone antagonist K sparing diuretic ACE-I/ ARB Adrenal insuff (Addison's dz) Congenital Adrenal Hyperplasia Renal tubular dysfx
123
When to clinical features of Hyperkalemia generally appear?
7 or greater "It's A F A C T"
124
A F A C T
Arrhythmia Flaccid paralysis Asc muscle weak Conduction abn T waves (tall and peaked)
125
Tx for Too much K | Hyperkalemia
``` ER/ICU if >6.5 Correct levels (rapid or slow) ```
126
Rapid correction of Hyperkalemia
IV Calcium Chloride Maneuver to shift K from ECF to ICF
127
Birmingham lecture | treat for too much K
Sodium bicarb IV | D50W + Insulin IV
128
Slow correction of Hyperkalemia
Loop or thiazide diuretic | Hemodialysis
129
Med cause of too much K
ACE-I and ARBs K-sparing diuretic Aldo Antag
130
Tx of Hyperkalemia
Thiazide or Loop diuretic
131
Not enough K | Hypokalemia
<3.5
132
When does low K become dangerous?
below 3.0
133
Causes of Not enough K | Hypokalemia
Inadequate intake GI tract loss Kidney loss Redistribution
134
When does a person usually suffer from Hypokalemia d/t inadequate intake?
If they are already on Thiazide or Loop diuretic which enhances K excretion
135
Common cause for K los
GI loss Upper GI: vomiting, NG suction (this causes Alkalosis which promotes Renal K loss, further worsening the problem)
136
Too much Aldosterone function can lead to
Low K Hypokalemia (i.e. Hyperaldosteronism and Cushing's syndrome)
137
Redistribution causes of Low K
Alkalosis Insulin administration B-adrenergic agonists
138
Hypokalemia sx
U CRAMP
139
U CRAMP with low K
U waves ``` Cramp Rhabdo and Resp failure Anorexia, n/v Musc weakness Paralysis ```
140
Having a hard time correcting low K?
check Mg level
141
Rapid correction of Low K
Cardiac monitor IV Potassium Cl (check K every 2-4 hrs)
142
Slow correction of Low K
orally
143
Causes of Hypokalemia
vomiting/gastric suction Thiazide/loop Alkalosis Insulin
144
Alkalosis causes
Low K
145
KOH dissolves host cells and bacteria, sparing:
Fungi and elastin
146
Tzank Prep
Herpes Virus Giemsa or Wright stain Giant multinucleated cells
147
What is more commonly used to diagnose HSV than Tzanck smear?
Immunofluorescencse
148
India Ink
Cyrptococci | large capsules that exclude the ink
149
Dark field microscopy
thin bacteria | T. pallidum (Syphilis)
150
Diagnosing Syphilis (T. pallidum) We use dark field microscopy bc Syphilis is
cannot be cultured gram (-) but too thin for staining Dark stain reveals spirochetes
151
Extra testing for Syphilis (other than dark field)
Veneral Dz Research Lab (VDRL): - CSF test - Fluorescent Trep Antibody Absorption FTA-ABS ALSO Rapid Plasma Reagin (RPR) -serum
152
Extra testing for Syphilis
VDRL (venereal) | RPR (rapid plasma)
153
Latex agglutination
CSF for Meningococcal | also serum, saliva, urine
154
Latex agglutination
detects pathogen specific antibodies/antigens
155
ELISA | Enzyme Linked Immunoassay
Detects antibodies in serum (used for many body fluids) May need to repeat testing bc it takes up to 2 wks for some immune responses to kick in
156
IFA | Indirect Immunofluorescence Assay
Detects antibodies | Primary test for ANA
157
NAAT/PCR
Detects small quantities of bacterial/viral DNA or RNA | faster than ELISA
158
AFD Acid Fast Bacilli stain
Tuberculosis rapid and cheap (mycobacterial culture is most sensitive and spec)
159
Only types of sterile C&S
CSF, pleural, pericardial, peritoneal, or synovial fluids
160
MIC Minimum Inhibitory Concentration
Lowest conc of Abx to inhibit the growth of an organism- achieved through microdilution
161
Intermediate results on Antimicrobial sensitivity mean
Organism is inhibited by MAXIMAL recommended Abx dose
162
Blood culture results
Prelim: 24 hours | Identification of org: 48-72 hours
163
When should blood cultures be repeated within 48 hrs of starting Abx?
``` Staph Aureus bacteremia Endocarditis Infection site w/limited penetration Persistent leukocytosis Prosthetic vascular grafts Pacemaker Resistant pathogens Unknown source of original bacteremia ```
164
Bacteremia
infection specifically in the blood
165
Thoracocentesis
Aspirate fluid from PLEURAL space
166
Paracentesis
Aspirate fluid from ABDOMINAL cavity
167
Transudative effusion
Intact vascular wall | d/t Pressure differences
168
Transudative effusion usually d/t
CHF Liver cirrhosis Nephrotic synd
169
Is further testing usually needed for fluid from Transudative effusions?
NO
170
Exudative effusion
D/t inflammation and Vascular wall DAMAGE
171
Common causes of Exudative effusion
Infection CA Inflammatory disorder (RA, Lupus) Trauma
172
Does fluid from exudative effusion usually require further testing?
YES
173
Normal values for Pleural fluid (around lungs) AND Peritoneal fluid (around abdomen)
50 mL Clear, serous, light yellow NO RBC WBC <300
174
Parapneumonic effusion (exudative) most common causes
Bacterial PNA Lung abscess Bronchiectasis
175
Second most common cause of Parapneumonic effusion (exudative)
Lung CA Breast CA Lymphoma
176
Hemothorax (exudative) effusion
RBCs in pleural space >100,000 Main causes: trauma, CA, PE
177
PE can cause either
transudative or exudative effusion
178
Chylous effusion (exudative)
Lymphatic duct disruption or impairment Cloudy, milky +Triglycerides and lipids
179
Causes of Chylous effusion (exudative)
Trauma | CA to induce lymphoma
180
CXR before Thoracocentesis
PA, lateral, lateral decubitus Ensure fluid is accessible by needle Check for fluidy (lays out when person lies down)
181
Parapneumonic effusion should be sampled if it meets any of the following
Layers out >25 mm Loculated Assoc w thickened parietal pleura on CT Clearly delineated by US
182
Contra for Thoracocentesis
Significant thrombocytopenia
183
Rules for Thoracocentesis
Do not remove >1L | Do not perform bilaterally
184
Pleural fluid- Transudative features
Protein ratio <0.5 LDH <0.6 Others: WBC <300, glucose levels equal, ph 7.4-7.4
185
Pleural fluid- Exudative features
Protein ratio >0.5 LDH ratio >0.6 Others: WBC >500, Glucose serum, pH lower
186
Pleural fluid analysis
Gross app, cell count, gram stain, protein, glucose, amylase, cytology, CEA carcinoembryonic, cultures, AFB
187
Why would pleural fluid have a pH of <7.3?
infection esoph rupture CA
188
Why would amylase be elevated in pleural fluid?
pancreatitis esoph rupture CA
189
Eisonophilia in pleural fluid
Parasite CA TB
190
Causes of Ascites
Liver cirrhosis Alc Hepatitis Acute Liver failure
191
Gold standard for dx Peritoneal Effusion
US
192
Abdominal paracentesis indication
New onset Ascites with or without: Fever, abd tender, AMS, hypotension, periph leukocytosis, worsening kidney fx, trauma, severe cirrhosis
193
Abdominal paracentesis contra
Coag abnormalities Small amt fluid Previous abd surgery Massive ileus w bowel/distension
194
Peritoneal fluid- Transudative features
Albumin gradient >1.1
195
Peritoneal fluid- Exudative features
Albumin gradient <1.1
196
LDH ratio >0.6 regarding Peritoneal fluid
Bowel perforation CA Infection
197
Amylase elev with peritoneal fluid
Pancreatic Bowel perforation CA Esoph rupture
198
Ammonia with peritoneal fluid
Ruptured or strangulated bowel
199
Bilirubin in peritoneal fluid
Bowel or biliary perforation
200
Urea/Cr
Possible bladder rupture | trauma
201
Spontaneous bacterial peritonitis SBP IMP to recognize and tx d/t high Mortality
Pts with Hepatic cirrhosis and Ascites Sx: ACUTE ONSET fever, chills, abd pain, rebound tender Labs: exudative effusion
202
Pericardial normal values
<50 mL Clear, straw colored NO RBC OR WBC
203
Overall diagnostic approach for Pericardial effusion
CBC, CMP, Trop, Thyroid fx, ANA/ESR/CRP, EKC, CXR, US, MRI or CT Pericardiocentesis only if necessary
204
When to do pericardiocentesis
Pericardial effusion with TAMPONADE | If fluid needed for dx and may change tx
205
Contra to pericardiocentesis
Coagulopathy | Uncooperative pt
206
CSF located b/w
Pia and Arachnoid mater in the Subarachnoid space
207
Lumbar puncture indications categories
Infection CA Multiple Sclerosis Cerebral/Subarachnoid Hemorrhage
208
Subcategories of infection indications for LP
Meningitis Encephalitis Abscess Neurosyphilis
209
Contraindications to LP
Increased intracranial pressure Vertebral degenerative joint dz Infection near LP site Pts on anticoags
210
IDSA guidelines to do CT before LP!!!!
P A N I C S ``` Papilledema AMS Neuro def Immunocomp CNS dz hx Seizure within past week ```
211
Try to draw two separate blood cultures immediately if CNS infection is concern
before starting Abx if possible
212
CSF normal values
``` Clear Pressure <20 RBC and WBC 0-5 Protein 15-45 Glucose 50-75 LDH <40 ```
213
Xanthochromia (yellow) CSF
Bilirubin | indicating lysis of RBC
214
Decreased pressure (hypovolemia)
dehyrdation shock nasal fracture w dural tear
215
Increased ICP
infection intra-cranial bleed tumor hydrocephalus
216
Neutrophilia in CSF
Bacterial/TB meningitis Abscess Subarach bleed Tumor
217
Macrophage in CSF
Fungal/ TB meningitis Subarach bleed Brain INFARCTION
218
Lymphocytosis in CSF
VIRAL, SYPHILIS, Fungal, or TB meningitis Multiple Sclerosis Guillian Barre synd
219
Multiple Sclerosis and Guillian Barre Syn
Lymphocytes in CSF
220
Eisonophilia in CSF
Parasitic meningitis | Allergic rxn
221
Xanthochromia usually present with
Subarachnoid hemorrhage but beware it takes 2-4 hours to occur
222
Oligoclonal gamma globulin bands in CSF
Multiple Sclerosis
223
Lymphocytes in CSF and | Ologiclonal gamma
Multiple Sclerosis
224
What to order with CSF analysis
everything with others PLUS: Lactic acid VDRL
225
Is Lactic Acid elevated in viral meningitis?
NO | only in bacterial/fungal
226
Glutamine in CSF
Hepatic failure
227
CRP C-reactive protein
bacterial meningitis
228
Syphilis test
VDRL or FTA-ABS
229
Toxoplasmosis eval for CSF
Geimsa/Wright stain | also used for herpes
230
CSF Tubes
1: Chemistry- Protein and glucose 2: Microbio- Gram stain, Acid fast stain, C&S, PCR 3. Heme- Cell count and diff 4. Hold
231
Intracellular ions
K phosphate and organic ions protein
232
Extracellular iions
Mainly Na | Cl also
233
Types of IVF
Crystalloids Colloids Blood
234
Types of Crystalloids (most commonly used)
Isotonic (often- dehydration and hypovolemia) Hypertonic (emergency) Hypotonic (maintenance) Dextrose (sugar)
235
What is used for IV boluses in dehydration and hypovolemia?
CRYSTALLOIDS NS (0.9%) Lactated Ringer Plasmalyte
236
Life threatening hyponatremia
CRYSTALLOID Hypertonic 3% NS life threat low Na with water excess Caution: CPM
237
Maintenance fluids
CRYSTALLOIDS Hypotonic NOT good for replacing volume deficits like dehydration or hypovolemia
238
Hypoglycemia
CRYSTALLOIDS | D5W/Dextrose
239
Colloids
more likely to expand Vascular compartment Used when crystalloids fail to sustain plasma volume d/t low osmotic pressure
240
Colloids use (not often)
Pt with burn or peritonitis can't hold onto the Crystalloid fluid given
241
DexTRAN and Hetastarch | Colloids, not often used
Operation room
242
Types of COLLOIDS
Albumin DexTRAN Hetastarch
243
Albumin (a Colloid)
Liver dz Peritonitis Burn "third spacing"
244
PRBCs
used WITH Crystalloids to expand intravascular volume
245
What fluids can you bolus?
NS, LR, Plasma lyte, PRBCs Hypovolemia (dehydration or acute blood loss)
246
What incriments do we bolus?
250mL,500mL, 1L
247
Maintenance IVD
maintain water and electrolyte balance in someone who isn't eating/drinking normally
248
What is generally used for Maintenance fluid?
D5/0.5 NS with 20 meq KCl
249
Maintenance IVF
"Kg method" for Normal adult patients 1500 for basically everybody, then once past 20kg, add 20 ml/kg/day
250
Children require less sodium than adults
Use 0.25 instead of 0.5 NS
251
NEVER BOLUS
K-containing IVF
252
Parkland formula
Burn patients How much total fl to give them? % burns x weight x 4 mL 8 hours: give 1/2 total amt 8 hours: 1/4 8 hours: 1/4
253
Parenteral means
not into mouth into blood in this case
254
When to consider TPN for adults vs children with anticipated or current inadequate energy intake by mouth
adults 7-10 days | children 3-7 days
255
Diuretics cause you to
lose K
256
ACE-I | ARBs cause you to
retain K | too much K