class #3- germ theory Flashcards

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1
Q

who created the first microscope?

A

Antoine Leevanhoak

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2
Q

what contributions did Louis Pasteur bring to the scientific world

A

-father of microbiology
-dispelled the idea of spontaneous generation
-pasteurization
-first to develop vaccines

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3
Q

what is spontaneous generation

A

the idea that life arises from nothing

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4
Q

how did pasteur prove that spon. generation was false

A

created s-shaped flasks that were in contact with air, but bacteria could not reach the broth at the bottom. i.e. proves that microbes cannot arise from non-living materials

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5
Q

what did Joseph Lister contribute

A

that washing surgical instruments is a good idea

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6
Q

how did Lister first clean instruments

A

used carbolic acid (phenol) to sterilize bad wounds and surgical tools to prevent infections

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7
Q

what is Semmelweis contribute

A

he said that hand washing was a good idea!

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8
Q

what is cadaverous poisoning

A

Poisonous particles form dead bodies, spreading disease in hospitals

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9
Q

how does Pasteur complete Semmelweis’s ideas

A

he concluded that doctors could spread disease through unsanitary hands

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10
Q

what is pasteurization

A

a way to prevent spoilage, heating liquid to kill bacteria

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11
Q

who was the first to discover that yeast were alive

A

pasteur

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12
Q

who was john snow and what was his contribution

A

-a physician and the first epidemiologist
-john snow removed the handle of the Broad Street Pump and saved lives

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13
Q

what was the Broad Street Pump case

A

-open sewage resulted in an outbreak of cholera
- first demonstration of fecal-oral disease

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14
Q

who was Jenner and what did he contribute

A

contributed to the first vaccines through the smallpox virus

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15
Q

how was the first vaccine created

A

through cowpox, jenner scraped skin of healthy people and rubbed scabs from cowpox on them, then these people were protected from smallpox

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16
Q

what are other examples of some of the first vaccines?

A

chicken cholera, the rabies vaccine

17
Q

who was robert koch

A

the rival of pasteur

18
Q

what did koch contribute

A

-he developed ways of growing bacteria in pure culture by using agar
-developed koch’s postulates as criteria for proving that a bacteria is responsible for causing disease
-developed first “toxoids”

19
Q

what are “koch’s postulates”

A

rigorous criteria, not always possible to meet: assumes that we can grow the bacteria & must be an animal model of infection available

20
Q

what are the four postulates***

A
  1. microorganism must be found in abundance in all organisms suffering from disease, and should not be found in healthy organisms
  2. the microorganism must be isolated from a diseased organism and grown in pure culture
  3. the cultured microorganism should cause disease in a healthy organism
  4. the microorganism must be reisolated from the inoculated host
21
Q

what did Shibasoburo and Yersin co-discover

A

Yersinia pestis, a gram negative bacteria

22
Q

what is Griffiths transformation experiment

A

was the first experiment suggesting that bacteria are capable of transferring genetic information through a process known as transformation

23
Q

in griffiths experiment, what is the significance of smooth bacteria

A

smooth bacteria make a capsule and are VIRULENT

23
Q

what is transformation

A

uptake of naked DNA

23
Q

what does living S strain result in

A

death (has living s strain)

23
Q

in griffiths experiment, what is the significance of rough bacteria

A

rough bacteria are AVIRULENT

23
Q

what does living R strain result in

A

life (no s strain)

24
Q

what does a mix of living r strain and dead s strain result in

A

death (has living s strain transferred to r strain)

25
Q

what does dead s strain result in

A

life (no living s strain)

26
Q

what did Oswald Avery contribute

A

haligonian- showed that DNA was the genetic material