Chronic Leukemias Flashcards
chronic lymphocytic leukemia is a neoplasm of _____
chronic lymphocytic leukemia: neoplasm of naive B cells
most commonly presents as leukemia, but can also present as small lymphocytic lymphoma when there is generalized lymphadenopathy
what is the most common chronic leukemia of adults (disease of older adults, with mean age of 70)
chronic lymphocytic leukemia (CLL)
[can also present as small lymphocytic lymphoma]
how do patients with CLL (chronic lymphocytic leukemia) present?
usually asymptomatic, incidental finding of elevated WBC count (>5K lymphocytes)
increased B lymphocytes on peripheral smear, smudge cells - leukemic cells are more fragile and get squished during peripheral smear
if symptomatic - fatigue, weight loss, early satiety, infections, lymphadenopathy, hepatosplenomegaly
how does CLL (chronic lymphocytic leukemia) disrupt normal immune function? (2)
what can these issues lead to?
decreased normal antibody production —> hypogammaglobinuria (not making antibodies) - susceptible to bacterial infections
increased AUTO-antibody production —> RBC/platelet destruction —> Warm AIHI, immune thrombocytopenia
when CLL becomes aggressive, it can undergo a “Richter transformation” to become…
what is a key sign of this?
as CLL (chronic lymphocytic leukemia) acquires more mutations, it can transform to diffuse large B cell lymphoma (about 10% of patients)
very poor prognosis
sign: patient with CLL presenting with rapidly enlarging lymph node
why does it make sense that CLL (chronic lymphocytic leukemia) tumor cells are monoclonal?
one progenitor cell acquires mutation, now all tumor cells are derived from this one —> monoclonal
what is distinct about the immunophenotype of CLL (chronic lymphocytic leukemia) tumor cells?
CLL is B cell disease, but…
both B (CD20) and T (CD5) cell markers are expressed!
along with surface Ig (IgM or IgM + IgD)
what does histological pathology of small lymphocyte lymphoma look like?
remember that small lymphocytic lymphoma is the alternative presentation of chronic lymphocytic leukemia (B cell disease)
histologically - a sheet/sea of small lymphocytes, loss of lymph node architecture
if patients with CLL are asymptomatic, they are only monitored closely (60% will never require treatment)
however, if they are symptomatic, the “drug of choice” is…
ibrutinib: Bruton Tyrosine Kinase small molecule inhibitor —> inhibits malignant B cell proliferation [IBrutinib = Inhibit B cells]
[remember that pre-B cell receptor activates BTK, allowing maturation to occur]
adverse effects: low cell counts, HTN, increased risk of skin cancer
what kind of cancer does this describe?
- patients frequently asymptomatic
- disrupts normal immune function (can cause warm AIHA, thrombocytopenia)
- smudge cells on peripheral smear
- B cells markers + CD5
- treat with Ibrutinib
CLL (chronic lymphocytic leukemia): most common chronic leukemia in adults (older adults, avg aged 70)
B cell disease but has CD5 T cell marker!
Ibrutinib: small molecule inhibitor of Bruton Tyrosine Kinase (required for malignant B-cell proliferation)
what kind of cancer Hairy Cell Leukemia? what mutation causes it?
named for histological presentation - cells look hairy
rare, mostly older men, very slowly progressing
B cell neoplasm with BOTH B (CD19, CD20 + monoclonal Ig) and T (CD25 + CD103) cell markers
due to GOF mutation in BRAF V600E (RAF - serine/threonine kinase, activates proliferation)
how does Hairy Cell Leukemia present?
pancytopenia (weak, fatigue, “dry tap”)
splenomegaly (expansion of red pulp)
LOW levels of monocytes (unusual infections - mycobacterium avium intracellulare)
what cell markers are important for identifying Hairy Cell Leukemia? (2)
- flow cytometry:
B cell neoplasm with BOTH B (CD19, CD20 + monoclonal Ig) and T (CD25 + CD103) cell markers - TRAP+ (Tartrate-resistant acid phosphatase) - seen via staining, resistant to acid
*note that CD103 mediates cell migration, typically only found on T and DC cells
how is Hairy Cell Leukemia treated, a chronic B cell leukemia? (2)
very slow developing, so watch and wait if symptoms are limited
treatment:
1. RAF inhibitors (cells carry BRAF V600E mutation)
2. purine analogs: Cladribine (2-CdA) or pentostatin - disrupt DNA synthesis
what kind of cancer does this describe?
- rare, slow developing
- pancytopenia + splenomegaly
- “dry tap” on bone marrow biopsy
- BRAF mutation
- TRAP+
- Rx: Cladribine
Hairy Cell Leukemia (chronic B cell)
all cells have BRAF V600E mutation (of RAF - serine/threonine kinase)
Cladribine: purine analog (looks like adenosine), first line of treatment