Chromotography Flashcards
stationary phase
a solid or liquid that the mixture can travel across. the more attracted a portion of the mixture is then the slower it moves
mobile phase
a liquid or gas that flows across the stationary phase and carries the components of the mixture with it. the more soluble the faster it moves
separation of a mixture depends on
the solubility in the mobile phase and the attraction to the stationary phase
attraction depends on
polarity
TLC stationary and mobile phase
stationary- polar silica gel
mobile- non polar liquid solvent
TLC method
pencil line 1cm
small drop of sample on line
stand plate in a beaker containing small amount of solvent (level must be below the pencil line)
cover beaker so solvent doesn’t evaporate
allow solvent to travel up
remove plate when solvent has travelled 1cm below top
let plate dry
spray with ninhydrin
TLC developing agent
ninhydrin
Rf
distance travelled by spot/ distance travelled by solvent front
column chromatography stationary and mobile phases
stationary- polar silica gel
mobile- non polar liquid solvent
solvent is known as what in column chromatography
eluent
gas-liquid chromatography stationary and mobile phases
stationary- thick viscous liquid (non-polar) or silica gel coated on lining of column
mobile- inert gas
what is recorded by gas-liquid chromatography
retention time
how to find the amount of each component in gas-liquid
the area under the peak on the graph