Chromotography Flashcards

1
Q

stationary phase

A

a solid or liquid that the mixture can travel across. the more attracted a portion of the mixture is then the slower it moves

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2
Q

mobile phase

A

a liquid or gas that flows across the stationary phase and carries the components of the mixture with it. the more soluble the faster it moves

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3
Q

separation of a mixture depends on

A

the solubility in the mobile phase and the attraction to the stationary phase

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4
Q

attraction depends on

A

polarity

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5
Q

TLC stationary and mobile phase

A

stationary- polar silica gel
mobile- non polar liquid solvent

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6
Q

TLC method

A

pencil line 1cm
small drop of sample on line
stand plate in a beaker containing small amount of solvent (level must be below the pencil line)
cover beaker so solvent doesn’t evaporate
allow solvent to travel up
remove plate when solvent has travelled 1cm below top
let plate dry
spray with ninhydrin

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7
Q

TLC developing agent

A

ninhydrin

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8
Q

Rf

A

distance travelled by spot/ distance travelled by solvent front

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9
Q

column chromatography stationary and mobile phases

A

stationary- polar silica gel
mobile- non polar liquid solvent

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10
Q

solvent is known as what in column chromatography

A

eluent

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11
Q

gas-liquid chromatography stationary and mobile phases

A

stationary- thick viscous liquid (non-polar) or silica gel coated on lining of column
mobile- inert gas

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12
Q

what is recorded by gas-liquid chromatography

A

retention time

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13
Q

how to find the amount of each component in gas-liquid

A

the area under the peak on the graph

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