Chromotography Flashcards
1
Q
What does protein separation in NATIVE PAGE depend on?
A
Size, density and shape
2
Q
What does protein seperation in SDS depend on?
A
Size
3
Q
What are the four types of chromatography?
A
Size exclusion, ion exchange, affinity and reverse phase
4
Q
What is size exclusion?
A
Small proteins enter beads and bigger ones stay in buffer
5
Q
What is ion exchange?
A
Proteins are separated according to net charge
6
Q
What is affinity chromatography?
A
Beads link to the molecule that the protein binds too
7
Q
What is reverse phases chromatography?
A
Form hydrophobic reactions, the least hydrophobic elute first
8
Q
What is chromatography?
A
Separates components of a mixture
