chromatography Flashcards

1
Q

Column chromatography

A

Vertical column packed with solid powdered substance - acts as stationary phase
A solvent containing the mixture being analysed is then added and moves down the column as the mobile phase

Varying affinity means they drain out of column at different times - can be taken as separate samples
Retention time is measured - the time it takes to drain out - allow molecules to be identified

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2
Q

Gas chromatography

A

Thin tubes is packed solid power substance which acts as a stationary phase
A high pressured gas is passed through the tube as a mobile phase
Method used to separate mixtures of volatile liquid which are fed in as vapours
Analysis machine records a retention time for each component molecule in the mixtures - used identify them

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3
Q

GC- MS

A

Gas chromatography and mass spectrometry

Allows for a more advanced level of molecule analysis
Separated using the gas chromatography
Then Mass spectra identifies them
Much faster and more accurate

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