Chromatography Flashcards
What is the stationary phase in Thin Layer Chromatography
Silica plate or Alumina Plate
What is the mobile phase in TLC
The solvent which the plate is suspended in
How are substances separated in TLC
Difference in solubility in the solvent and the difference in affinity to the stationary phase
What is recorded for TLC
The Rf value
Advantages for TLC
- quick
- low tech
- very small samples can be tested
-cheaper
Disadvantages for TLC
Substances may not dissolve well in the solvent
Substances could have similar Rf values
What is used for the stationary phase in column chromatography
Silica or alumina plate or powder in a glass column
What is the mobile phase in column chromatography
The solvent poured through the column
How are substances separated in column chromatography
Difference in solubility in the solvent and difference in affinity to the stationary phase
What is recorded for column chromatography
Nothing
Advantages of column chromatography
Can use larger quantities
Low tech
Disadvantages of column chromatography
Rf values not calculated
Same substance can come out of the column at the same time
Stationary phase in gas chromatography
Silica or alumina coated within metal column
Mobile phase in gas chromatography
Carrier gas, N2, He
How are substances seperated in gas chromatography
Differences in affinity to stationary phase
