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Chemistry > Chromatography > Flashcards

Chromatography Flashcards

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1
Q

principle of chromatography

A

adsorption or partition

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2
Q

column chromatography

A

stationary phase is solid or liquid supported by a solid

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3
Q

thin layer chromatography

A

used to separate mixtures, check purity of a mixture and to monitor the progress of reaction

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4
Q

paper chromatography

A

liquid liquid chromatography can also be carried out on a strip of paper. thin liquid film of solvent held on the paper will act as a stationary phase.

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5
Q

adsorption CC

A

column of solid is used as adsorbent

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6
Q

partition CC

A

when solid column is acting only as a support to the liquid adsorbent

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7
Q

chromatogram

A

a banded column of adsorbed constituents

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8
Q

elution and eluent

A

dissolving out of component s form adsorbent is called elution and the solvent is called eluent

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9
Q

applications of CC

A

1) quantitative separation
2) purification
3) concentration
4) identification

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10
Q

chromatoplate

A

glass plate with thin layer of stationary phase

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11
Q

how do you get the result if the components are colourless in TLC

A

the dried plate is sprayed with a suitable reagent to make the spot coloured

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12
Q

Rf value

A

the ratio of distance travelled by a component to the distance travelled by solvent front

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13
Q

advantages of TLC

A

1) separation of minute quantities
2) sensitive and sharp separation
3) high speed of separation
4) wide choice of stationary phases

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14
Q

applications of TLC

A

1) detection of purity and identification
2) pharmaceutical, food and cosmetic industry
3) biochem analysis

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15
Q

HPLC

A

Modern method and powerful tool for the separation, purification and identification of various non volatile high molecular weight organic compounds and natural products

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16
Q

advantages of HPLC

A

1) high speed separation
2) excellent column separation
3) solvent consumption is minimum
4) sensitive and accurate
5) can be done in ambient temp

17
Q

2 types of detectors in HPLC

A

1) bulk property

2) solute property

18
Q

2 ways in which elution can be done

A

1) isocratic done by 1 solvent of constant composition

2) gradient done by 2 or more solvents differing in polarities

19
Q

applications of HPLC

A

1) quality control
2) process control
3) forensic analysis
4) environmental monitoring
5) clinical testing
6) biomed research work

20
Q

gas chromatography

A

for separation and analysis of gaseous mixture and volatile organic compounds

21
Q

applications of gas chromatography

A

1) analysis of natural gas, gasoline plant samples, refinery gases, synthetic rubber and plastic intermediates, steroidal hormones
2) separation of close boiling liquids
3) determination of ethyl alcohol content in blood using glycol column
4) multicomponent analysis
5) steroidal hormones

22
Q

analyte

A

substance to be separated during chromatography

23
Q

bonded phase

A

stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing

24
Q

chromatogram

A

visual output of chromatograph

25
Q

chromatograph

A

enables sophisticated separation

26
Q

chromatography

A

separation that distributes components to separate between 2 phases, one stationary while other moves in definite direction

27
Q

eluate

A

mobile phase leaving column

28
Q

eluent

A

solvent that carries analyte

29
Q

eluotropic series

A

list of solvents ranked acc to their eluting power

30
Q

immobilized phase

A

stationary phase that is immobilized on the support particles or on inner wall of column tubing

31
Q

preparative chromatography

A

purify sufficient quantities for further use

32
Q

retention time

A

time taken for analyte to pass through the system

33
Q

sample

A

matter analyzed

Chemistry (10 decks)

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