CHOLERA

Question 1

What causes cholera

Answer 1

vibrio cholerae bacterium

Question 2

how does vibrio cholarae cause disease cholera

Answer 2

the bacterium produces toxin which can cause profuse watery diarrhoea and vomiting and death due to dehydration, if left untreated

Question 3

transmission of vibrio cholerae

Answer 3

primarily by drinking water or eating food that has been contaminated by the feces

Question 4

explain the morphology of vibrio cholerae

Answer 4

1. Short, curved or comma shaped
2. Gram negative rod with rounded or pointed ends
3. actively motile with a single polar flagellum
4. non sporing
5. Non-capsulated
6. Non acid fast

Question 5

what are the culture characteristics of vibro cholera

Answer 5

1. strongly aerobic
2. Optimum temperature for its growth is 37 c
3. PH is 6.4 -9.6 favours better growth
4. NaCl conc. 0.5- 1.0 %
5. It is quite sensitive to drying, exposure to sunright and extreme changes in PH
6. It is also inhibited by normal intestinal flora

Question 6

result of cuturing cholera on macconkey agar

Answer 6

shows colourless colonies

Question 7

result of blood agar culture on cholera

Answer 7

beta hemolysis

Question 8

what transport media is used to transport cholera sample to lab

Answer 8

Venkata raman (VR) MEDIA

Question 9

what selective media is used in cholera vibrio culture

Answer 9

Thiosulphate citrate bile and sucrose (TCBS)

Question 10

what enrichment media is used

Answer 10

alkaline peptone water, taurocholate peptone water

Question 11

what planting media is used

Answer 11

alkaline bile salt agar medium, monsur galatin taurocholate trypticase tellurite agar medium.