CHEM NOTES TO KNOW

Question 1

What is the molecular formula for pyrrole?

Answer 1

c4H5N

Question 1

what is the formula for Fluid pressure?

Answer 1

P = pgh
(p= density ; g= gravitational acceleration; h= height )

Question 2

What are the conditions for STP ? AT STP, how many liters is 1 mole of gas?

Answer 2

STP : 1 atm and 0 degrees celsius
1 mole of gas = 22.4 L (or 2.24 x 10^4 cm^3)

Question 3

What is a major characteristic of amino acid residues?

Answer 3

Amino acid residues DO NOT HAVE CHARGE

Question 4

What occurs during proteolysis ?

Answer 4

The number of lower molecular weight bands increases with time (bottom); while the number of higher molecular weight bands diminishes over time

Question 5

what is affinity chromatography?

Answer 5

A separation method based on a specific binding interaction between immobilized ligand and its binding partner

Question 6

What is the function of detergents?

Answer 6

to isolate membrane proteins; by having a combination of large hydrophilic and hydrophobic regions

Question 7

What is ki in kinetics?

Answer 7

Ki: the concentration of an inhibitor at which reaction rate is half of max reaction rate

Question 8

What does a positive vs negative Hill coefficient suggest?

Answer 8

Hill coefficient of greater than 1 mean positive cooperative (substrate binds to somethings, makes it EASIER for others to bind.
negative coefficient (< 1) means negative cooperatively, make it DIFFICULT for others to bind

Question 9

What is needed to form salt bridges between amino acid residues?

Answer 9

You need a positively charged side chain of one Amino acid and a Negatively charged side chain of another amino acid residue

Question 10

What is needed for pi stacking interactions?

Answer 10

You need an amino acid residue with aromatic ring

Question 11

What are the acidic amino acids and what charge do they have?

Answer 11

Acidic amino acids: glutamate and Aspartate
have positive charge

Question 12

What are the Basic amino acids and what charge do they have?

Answer 12

Basic amino acids: Arginine, histidine, lysine)
have Negative charge

Question 13

What is the formula for formal charge?

Answer 13

Formal charge = valance e- - sticks - lone pairs

Question 14

What is the hybridization for Deuterium?

Answer 14

Deuterium is an isotope of hydrogen and 1s orbital. hence it is not hybridized. (just s for each deuterium atom)