Chapter 8: Microbial Metabolism Flashcards
The sum of chemical reactions in organism
Metabolism
Provides energy and building blocks for anabolism
Catabolism
Uses energy and building blocks to build large molecules
Anabolism
Uses light energy and CO2 as primary carbon source; photosynthesis
photoautotrophs
Use light energy but cannot convert CO2 to sugar
Photoheterotroph
Energy used in Calvin -Benson Cycle to fix CO2
Chemoautotroph
Use electrons from hydrogen atoms in organic compounds as their energy source
Chemohetetrotrophs
Removal of electrons
Oxidation
Gain of electrons
Reduction
Factors that affect enzyme activity
Temperature, pH, substrate concentration
What happens in glycolysis?
Glucose is split in half and 2 ATP is produced
Is glycolysis catabolism or anabolism
Catabolism
Energy from the transfer of high-energy PO4 - to ADP generates ATP
Substrate- level phosphorylation
Energy released from the transfer of electrons from 1 compound to another is used to generate ATP in ETC
Oxidative Phosphorylation
Final electron acceptor is O2
Aerobic respiration
Final electron acceptor is not O2
Anaerobic respiration
How is anaerobic respiration different from aerobic respiration?
Anaerobic respiration DOESN’T use O2 the final electron acceptor but aerobic respiration does
How many ATPs do Prokaryotes generate from the aerobic breakdown of 1 molecule of glucose?
38 ATP
Urease biochemical test
Oxidase biochemical test
Fermentation test
Producing urease
Used to detect cytochrome c oxidase
They can ferment a specific carbohydrate
What happens in the krebs cycle ?
Oxidation of Acetyl CoA using decarboxylation and redox reaction
What happens in the ETC cycle and chemiosmosis ?
In ETC, a series of carrier molecules that are oxidized and reduced as electrons are passed down chain.
In chemiosmosis, it is using released energy to produce ATP.
The different types of fermentation
Alcohol and lactic acid fermentation