Chapter 8 - HPLC maintenance and troubleshooting Flashcards
1
Q
Frequently encountered troubleshooting categories (4)
A
- pressure
- baseline
- peak
- data
2
Q
The typical wearable item in the autosampler is the
A
injector rotor seal.
3
Q
A rotor seal can last approximately how many injections?
A
30,000
4
Q
Verify that a problem exists by
A
repeating the experiment.
5
Q
Baseline drift in a UV detector often reflects
A
the change of the energy output of the UV lamp with time.
6
Q
Spikes on the baseline are likely caused by
A
air bubbles out-gassing in the detector flow cell.
7
Q
Peak problems such as poor resolution, broad, split, tailing, or fronting peaks, and extra peaks are most often caused by
A
the column.
8
Q
Fronting peaks are caused by
A
column overload.
9
Q
OOS stands for
A
Out of specification
