Chapter 7 - HPLC operation guide

Question 1

By convention, is A or B the weak mobile phase?

Answer 1

Mobile phase A.

Question 2

pH adjustments should be made in which mobile phase?

Answer 2

MPA.

Question 3

Store RPC columns in

Answer 3

ACN, MeOH, or a mixture of water and organic solvents.

Question 4

Unused columns should be

Answer 4

capped with closed fittings.

Question 5

Buffers should not be left sitting in the column due to the

Answer 5

chance of precipitates forming.

Question 6

Typical column injections in lifetime

Answer 6

1000-3000

Question 7

Column performance decreases with time as signified by

Answer 7

increased back-pressure and peak widths.

Question 8

UV/Vis or PDA should be turned on for how long before use?

Answer 8

15 minutes

Question 9

Standard spectral resolution

Answer 9

2-4 nm

Question 10

Deuterium UV lamps typically last

Answer 10

12 months or 1000-2000 hours.

Question 11

In HPLC, buffered mobile phases are usually pumped out with

Answer 11

10% MeOH in water.

Question 12

For RPC, at least __________ column void volumes of the initial mobile phase must be used to equilibrate the column.

Answer 12

3 to 5

Question 13

Airtight vials should be avoided to

Answer 13

eliminate the possibility of a partial vacuum during sample withdrawals.

Question 14

Number of points per peak should be at least

Answer 14

10-20

Question 15

Goal of quantitative analysis

Answer 15

to obtain a chromatogram with adequate separation of key analytes.

Question 16

Calibration is

Answer 16

the process of establishing a calibration curve of the specified analyte from a set of injected calibration standard solutions.

Question 17

Reporting is

Answer 17

the process of generating a formatted report from the result files using a reporting method.