Chapter 7 ( Techniques Of Genetic Analysis )

Question 1

How to visualize all the DNA fragments on a gel ?

Answer 1

Using ethidium bromide

Question 2

Fragile X syndrome ?

Answer 2

The leading cause of inherited mental retardation
Associated with large ears , elongated face and hypermobile joints
The gene involved is FMR1

Question 3

Uses of reverse transcriptase PCR ?

Answer 3

Detecting RNA viruses such as HIV ( measuring the viral load in AIDS patients in this way can be used to monitor the state of infection and response to antiviral drugs )
Determine whether a gene is transcribed

Question 4

Whats the use of Reverse transcriptase PCR in CML patients ?

Answer 4

To monitor the level of BCR-ABL mRNA during therapy with Imatinib which is helpful for both diagnosis and management of therapy

Question 5

BCR-ABL protein ?

Answer 5

Abnormal fusion protein with tyrosine kinase activity is found in CML patients due to the presence of Philadelphia chromosome translocation between chromosome 9 and 22