Chapter 6 ( Genetic Strategies In Therapeutics )

Question 1

Function of restriction endonucleases in bacteria ?

Answer 1

Protects the bacteria from infection by DNA viruses

Question 2

Palindromes ?

Answer 2

Sequence of DNA that can be read same backwards and forwards usually of 4-8 base pairs in length on which a restriction endonuclease works

Question 3

Examples for recombinant proteins ?

Site of their production ?

Answer 3

Insulin —- E. coli
Growth factor —- E. coli
Epidermal growth factor —— E. coli 
Hepatitis B vaccine—— Saccharomyces cerevisae
Erythropoietin ——- mammalian cells 
Factor Vlll—— mammalian cells

Question 4

Advantages of adeno assotiated viruses in gene delivery ?

Answer 4

No disease association
Limited innate immunity
Restricts expression to specific tissues

Question 5

Example for in vivo gene replacement therapy ? Whats the viral delivery vehicle ?

Answer 5

Gene replacement therapy for cystic fibrosis

Adeno-associated viruse

Question 6

Example for ex vivo gene replacement therapy ? What’s the clone delivery vehicle ?

Answer 6

Gene replacement therapy for X-linked SCID

Retrovirus with IL-R gamma chain gene

Question 7

Aspects of use of RNA interference technology ?

Answer 7

Ttt of cancer
Ttt of many neurodegenerative diseases
Ttt of age-related macular degeneration