Chapter 5 ( Regulation Of Eukaryotic Gene Expression

Question 1

Transcription factors domains ?

Answer 1

DNA binding domain

Activation domain

Question 2

Steroid receptors ?

Answer 2

Specific transcription factor
Binds to HRE
Leading to steroid response
Protein class : zinc finger

Question 3

cAMP response element binding protein ( CREB protein ) ?

Answer 3

Specific transcription factor
Binds to CRE
Leads to response to cAMP
Protein class : leucine zipper

Question 4

Peroxisome proliferator-activated receptors ( PPARs ) ?

Answer 4

Specific transcription factors
Binds to PPREs
Leading to regulation of multiple aspects of lipid metabolism
Activated by Fibrates and Thiazolidinediones
Protein class : zinc finger

Question 5

Nuclear factor kappa-B (NFkB) ?

Answer 5

Specific transcription factor
Binds to kB elements
Leads to regulation of expression of many genes in immune system
Protein class : Rel domains

Question 6

Homeodomain receptors ?

Answer 6

Specific transcription factor
Made by homeobox gene/ PAX gene
Binds to same enhancer for different genes
Lead to regulation of gene expression during development
Protein class : helix-turn-helix

Question 7

Mutation in homeobox gene/PAX gene ?

Answer 7

Klein Waarderburg syndrome

Question 8

Zellweger syndrome ?

Answer 8

Genetic disease caused by mutation in genes involved in peroxisome biogenesis
Leads to deficiency of peroxisomes and accumulation of very long chain fatty acids
Features : enlarged liver , high serum Cu and Fe and vision problems
Symptoms : growth and mental retardation
Abnormal muscle tone
Death in first year

Question 9

Hormone receptors that are zinc finger proteins ?

Answer 9

All steroid hormones
Thyroid hormones
Vitamin A,D

Question 10

Klein-Waardenberg syndrome ?

Answer 10

Caused by mutation in PAX gene 
Symptoms: limb abnormalities 
dystopia canthorum 
Pigmentary abnormalities 
Congenital deafness

Question 11

Holoprosencephaly ?

Answer 11

Failure of the brain to separate into distinct right and left halves

Question 12

Upstream promotor elements examples ?

Answer 12

1- CCAAT box binds general transcription factor NF-1
2- GC-rich sequence binds general transcription factor SP-1
3- TATA box binds general transcription factor TFllD with its TATA box binding protein (TBP)