Chapter 7 physical and chemical methods of sterilization

Question 1

use of chemical agents on living tissue to prevent spread of microbes

Answer 1

Antisepsis

Question 2

kills bacteria

Answer 2

bactericidal

Question 3

Inhibits bacteria

Answer 3

Bacteriostatic

Question 4

most microbial forms on inanimate objects are killed without necessarily killing them

Answer 4

Disinfection

Question 5

destroys spores, fungi, and viruses

Answer 5

Sporicidal, fungicidal,viricidal

Question 6

most common method of sterilization

Answer 6

Heating

Question 7

moist heat has greater killing action than dry heat

Answer 7

Nature of heat

Question 8

Preferred over dry heat

Answer 8

moist heat

Question 9

This methods destroys disease causing microbes in milk

Answer 9

Pasteurization

Question 10

is used to destroy contaminating bacteria in vaccine preparations

Answer 10

Vaccine bath

Question 11

used to inactivate bacteria in Serum preparations

Answer 11

Serum bath

Question 12

to solidify and disinfect egg containing and serum containing media

Answer 12

Inspissation

Question 13

boiling water at 100C

Answer 13

Boiling

Question 14

involves exposing material to live steam at 100C for 30-90 minutes for 3 consecutive days

Answer 14

Tyndallization

Question 15

most efficient method of sterilization

Answer 15

Autoclave

Question 16

This method is used to sterilize articles like bacteriological wire loops, straight wires, tips o forceps, and searing spatulas. The materials are held over a bunseen burner until they become red hot

Answer 16

Dry heat

Question 17

The material is passed over the flame several times but is not heated to redness

Answer 17

Open flame

Question 18

Burning organism into ashes

Answer 18

incineration

Question 19

placed in oven with a temp of 160C for over 1 hour

Answer 19

hot air oven

Question 20

Are phenol derivatives that are more potent and safer than phenol

Answer 20

Cresols

Question 21

Is used as a skin disinfectant if in isopropanol solution

Answer 21

Chlorhexidine

Question 22

used for topical purposes

Answer 22

Chloroxylenols

Question 23

Is a chlorinated diphenyl which has a greater activity against gram positive bacteria

Answer 23

Hexachlorophene

Question 24

An organic phenyl ether

Answer 24

Triclosan

Question 25

disorganize the lipid structure of the cell membrane. Skin irritants and flamable

Answer 25

Alcohol

Question 26

Used as a skin atiseptic

Answer 26

Ethyl alcohol

Question 27

has greater bactericidal acitvithy than ethyl alcohol and is less volatile

Answer 27

isopropyl

Question 28

used mainly as preservative

Answer 28

Benzyl

Question 29

Fungicidal and sporicidal

Answer 29

methyl