Chapter 7-Complementation Flashcards
No complementation
When both parents carry a mutation on the same gene. F1 generation will show mutant geneotype
complementation
mutations are on different genes therefore F1 generation will not show mutant phenotype
complementation test
used to determine whether two mutations that cause the same phenotype are mutations in the same gene or in different genes. Can only be used for recessive mutations
auxotroph
an organism, such as a strain of bacteria, that has lost the ability to synthesis certain substances required for its growth and metabolism
prototroph
a wild type cell that can grow on minimal medium alone
Transition
When one purine (A or G) replaces the other purine
Base substitution
When a base at a certain position in one strand of the DNA molecule is replaced by one of the other three bases
Transversion
When a purine changes to a pyimidine or vice versa
Inversion
A 180 degree rotation of a segment of DNA
Translocation
Parts of two non homologous chromosome change places
Deletion
Occurs when a block of one or more nucleotide pairs is lost from a DNA molecule
Insertion
The addition of one or more nucleotide pairs
Deamination
The removal of an amino acid group. Can change cytosine to uracil.
Intercalator
Class of chemical mutagens composed of flat planar molecules that can sandwich themselves between successive base pairs and disrupt the machinery of replication, recombination, or repair
Unequal crossing over
Change in DNA caused by erroneous recombination in which one homologous chromosome end up with a duplication while the other homologe sustains a deletion