Chapter 11&9 Flashcards
DNA polymorphism
A DNA sequence that occurs in 2 or more variant forms
haplotype
combination of alleles at multiple tightly linked loci that are transmitted together over many generations
RFLP (restriction fragment length polymorphism)
variation in the length of a restriction fragment detected by a particular probe due to nucleotide changes at a restriction site
ASO (allele specific oligonucleotides)
a short oligonucleotide pride that will hybridize to only one allele at a chosen SNP locus
SNP (single nucleotide polymorphism)
two different nucleotides appear at the same position in genomic DNA from different individuals
DNA fingerprinting
detection of genotype at a number of unlinked highly polymorphic loci using one probe
locus
location on a chromosome
microsatellite
repeating sequence of 4 or more base paris of DNA (CACACA). Tend to occur in noncoding DNA
vector
a DNA molecule used for transporting, replicating, and purifying a DNA fragment
genomic library
a collection of the DNA fragments of a given species inserted into a vector
cDNA
DNA copied from RNA by reverse transcriptase
hybridization
stable binding of single stranded DNA molecules to each other
PCR
efficient and rapid technique for amplifying the number of copies of a DNA fragment
reverse translation
determining the possible sequence of base pairs associated with a particular region of a polypeptide
recombinant DNA
contains genetic material from two different organisms
