Chapter 7

Question 1

Inactivation

Answer 1

destroys complement activity

Question 2

Complement

Answer 2

Interferes with syphilis tests and complement components. Agglutinates latex particles, causing false positives in latex passive agglutination assays

Question 3

inactivation of complement in serum

Answer 3

Heat at 56 degrees Celsius for 30 minutes

Question 4

Re-inactivation of complement in serum

Answer 4

Heat at 56 degrees Celsius for 10 minutes, when greater than 4 hours has elapsed since inactivation

Question 5

Acute phase of illness

Answer 5

when the disease is first discovered or suspected

Question 6

Convalescent phase of illness

Answer 6

about 2 weeks after acute phase

Question 7

antibody titer

Answer 7

the reciprocal of the highest dilution of the patien’s serum in which the antibody is still detectable. read at the highest dilution that gives a positive reaction with the antigen

Question 8

simple Dilution formula

Answer 8

Dilution = solute or sample volume (SV)/Total volume (TV)

Question 9

Total volume of dilution

Answer 9

Solute or sample volume + diluent volume

Question 10

Dilutions vs Ratios

Answer 10

ARE NOT THE SAME. 1:4 dilution = 1:3 ratio of sample to diluent

Question 11

Dilution sample: 5ml of 1:10 dilution is needed. How much serum and diluent are needed for this dilution?

Answer 11

TV is 5ml. 1/10 = x/5ml 5ml = 10x x= 5/10, or 0.5 mL (1/2 of 1mL) Diluent volume = 4.5ml