Chapter 6-7 Quiz Flashcards
Light microscopes
The advantage is being able to view dynamic processes in living cells… ie; contractile vacuole seen pumping out water of paramecium in pond water
The limitation of the LM is that the shortest wavelength of light limits the solving power of the LM.
Electron microscopy
There are two kinds
1- scanning electron microscopes: show surface details by focusing a beam of electrons onto the surface of a specimen providing 3D images
2- transmission electron microscopes: show internal details also by focusing a beam of electrons through a specimen.
Mainly used for the study of internal structure of cells, cannot see dynamic processes
Cell fractionation: purpose and method
Cell fractionation takes cells apart and departed the major organelle for their specific functions can be determined. A homogenizer is like a blender, then centrifuge process keeps spinning and collecting fractions of light material as the heavier material sinks to the bottom. The heavier material is called the pellet. The light ones remain in the supernatant.
Helps correlate cell function with structure
Prokaryotes
Simpler and smaller than eukaryotes
Have no nucleus or endoplasmic reticulum
DNA is in unbound region called nucleoid
Non membrane bound organelles means that the basic cell functions are different in prokaryotes than eukaryotes
Bacteria
Eukaryotes
Have DNA in nucleus
Complex and larger than prokaryotes
Membrane bound organelles
Plants animals fungi are examples
The nucleus
Contains DNA and protein in the form of chromatin which are long and fiber like
During cell division the DNA condenses into discrete unites called chromosomes
The nucleolus is located within the nucleus and is the site of ribosomal RNA (rRNA) synthesis
Ribosomes
Complexes of rRNA and protein
Carry out protein synthesis
Free ribosomes are in the cytosine
Bound ribosomes are on the outside of the endoplasmic reticulum or the nuclear envelope
Contractile vacuole
Found in many freshwater protists, pump excess h20 out
Endoplasmic reticulum
Smooth- lacks ribosomes, synthesizes oils phospholipids and steriods, metabolizes carbs, detoxifies drugs and poisons, stores calcium ions
Rough- site of synthesis of proteins that are exported from the cell. Has ribosmesbthatvsecrete glycoproteins. Distributed transport vesicles, secretory proteins surrounded by membranes. Is a membrane factory for the cell
Golgi apparatus
Warehouse
Transport molecules due to thei polar characteristics
Constituted of flattened membrane sacs called cistae
Lysosomes
A membranous sac I. Which hydrophilic enzymes that can cause collateral damage are packaged to prevent general destruction of cellular components
Autophagy
When lysosomes eat their own organelles and macromolecules
Vaculoes
Maintenance
3 types:
Food, contractile, and central
Food vacuoles are formed by phagocytosis
Central vacuoles hold reserves of organic compounds
Mitochondria
Powerhouse
Only in eukaryotes
Have Cistae that increase the surface area for enzymes that synthesize ATP
Chloroplasts
Capture light energy AKA PLASTIDS photosynthesis- light to chem energy Thylakoids ante membranous sacs that are stacked for form granum Stroma is interns fluid of chloroplasts