Chapter 6 Flashcards
Biologists use ___ and ___ to study ___
microscopes, biochemistry, cells
Cell walls were first seen by
Robert Hooke
Who first saw living cells?
Antoni van Leeuwehoek
light microscope (LM)
visible light passed through specimen and glass lenses; lenses refract light to magnify image
3 parameters for microscope
magnification, resolution, contrast
magnification
ratio of object’s image size to real size
resolution
clarity of image; min distance 2 points can be separated and still be distinguished as separate points
contrast
difference in brightness between light/dark areas
organelles
membrane-enclosed structures within eukaryotic cells
electron microscope (EM)
focuses beam of electrons through specimen/onto surface
resolution is inversely related to wavelength of light/electrons
scanning electron microscope (SEM)
detailed study of topography of specimen
electron beam scans surface of sample, which excites electrons on the surface and they’re detected by a device that translates the pattern of electrons into an electronic signal
types of LM
brightfield (stained/unstained), phase contrast, differential-interference-contrast (nomarski), fluorescence, confocal, deconvolution, super-resolution
types of EM
SEM and TEM
transmission electron microscope (TEM)
study internal structure of cells
aims electron beam through very thin section of specimen
use electromagnets as lenses to bend paths of electrons
cytology
study of cell structure
biochemistry
study of chemical processes (metabolism) of cells
cell fractionation
technique for taking cells apart and separating major organelles and sub cellular structures from one another
uses centrifuge which spins around test tubs w/ mixtures of disrupted cells at series of increasing speeds
resulting force causes subset to settle to the bottom of tube
enables bulk of a specific cell component
___ cells have internal membranes and ___ their functions
eukaryotic, compartmentalize
cytosol
semifluid, jellylike substance, in all cells, subcell components suspended in
chromosomes
carry genes in DNA form