Chapter 4: Cellular Metabolism Flashcards
The capacity to do work
Cellular Metabolism/Energy
Two types of energy
Energy of position and energy of motion
Potential energy and stored energy is a form of….
Energy of position
Active energy and energy in motion is a form of…
Energy of motion
One form of potential energy (energy of position)
1) Chemical (CPE)
Energy stored in the bonds of chemical substances
Chemical energy
Energy released from nuclear fission or nuclear fusion
Nuclear energy
Five types of active energy (energy in motion)
Kinetic, light, heat, electrical, sound
Work needed to accelerate a body of mass
Kinetic energy
Energy traveling in light waves (I e, visible light, ultraviolet light, x-rays)
Light energy
Random motion of microscopic particles of matter
Heat energy
Bulk flow of charged particles
Electrical energy
Mechanical vibrations known as sound waves
Sound energy
ATP (chemical energy)
Chief “currency” in all cells as source of immediate energy
What is ATP composed of?
Ribose, adenine, chain of three phosphates
5 carbon sugar
Ribose
Nitrogenous base
Adenine
Key to energy storage
Chain of three phosphates:
AMP, ADP, ATP
A nucleotide that plays a key role in many cellular processes, including energy metabolism, signaling, and RNA synthesis:
Adenosine monophosphate (AMP)
A molecule crucial for energy transfer within living cells, essentially acting as a precursor to ATP (Adenosine Triphosphate) by accepting a phosphate group to store energy when needed; it consists of an adenine base, a ribose sugar, and two phosphate groups.
Adenosine diphosphate (ADP)
A molecule considered the primary energy currency of cells, meaning it stores and provides readily usable energy for various biological processes within living organisms; essentially, it’s the “fuel” that cells use to function.
Adenosine triphosphate (ATP)
True or false: every ATP molecule cycles through an ATP cycle reaction (ATP>ADP + P) 5,000 times every 24 hours
True
True or false: ATP is not suitable for long-term energy storage
True
True or false: cells store only a few seconds worth of ATP
True
True or false: fat’s in carbohydrates are used for long-term
True
The ATP cycle:
ATP is enzymatically broken down to ADP + Pi (inorganic phosphate). Then ADP + Pi is converted back to ATP
Starch ATP
Plants
Glycogen ATP
Animals
Organisms that make their own food from inorganic carbon sources such as CO2 and energy from the environment
Autotrophs (producers)
Examples of autotrophs
Plants, algae, many bacteria
Organisms that obtain energy and carbon from organic compounds assembled by other organisms
Heterotrophs (consumers)
Examples of heterotrophs
Animals, fungi, many bacteria
True or false: ultimately, most life forms get their energy from the Sun (heat)
True
True or false: plants use photosynthesis to capture sunlight to make (sugars) starch.
True
True or false: herbivores eat the plants for energy
True
True or false: carnivores eat the herbivores for energy
True
True or false: decomposition of plant and animal material contributes to the nutrient pool.
True
What life does not rely on the Sun?
Chemolithrophic or chemo autotrophic prokaryotes: (ie., microbes in Hot springs and deep sea organisms, extremophiles (food through chemical process).
First law of thermodynamics
Total amount of energy in the universe remains CONSTANT, energy cannot be created or destroyed, energy can only CHANGE FROM ONE FORM TO ANOTHER, during conversion some energy is lost as HEAT. (Chemical>kinetic>heat)
Second law of thermodynamics
Entropy (disorder) is continuously increasing, energy transformations proceeds spontaneously to convert matter from a more ordered/less stable form to a less ordered/more stable form, a spontaneous process is one that occurs without intervention
True or false: spontaneous disorder occurs without any intervention or energy (less ordered/more stable form)
True
True or false: order requires energy to convert matter from a more ordered/less stable form
True
True or false: spontaneous does not mean instantaneous
True
Physical or chemical change that occurs by itself or without intervention from an outside source
Spontaneous process
Examples of spontaneous process:
An iron object will rust when left in moist air; heat from a hot object will flow to a cold object; a zorb at the top of a hill will roll down the hill
True or false: examples of spontaneous process will occur without requiring an outside force and continue until equilibrium is reached
True
This is comprised of catabolism and animalism
Metabolism
The process of breaking down
Catabolism
The process of building up
Anabolism
These reactions break down, hydrolysis, decomposition
Catabolic reactions
These reactions build up, dehydration, synthesis
Anabolic reactions
Catabolic reactions
Glycogen >< glucose, protein ><amino acids, triglyceride><fatty acids, RNA><nucleotides
Anabolic reactions
Glucose><glycogen, amino acids- enzyme, fatty acids><phospholipid, nucleotides-DNA
True or false: all chemical reactions are either exergonic or endergonic
True
A net release of energy
Exodonic reactions
Give off heat
Exothermic
Net input of energy
Endergonic reactions
Absorbs heat
Endothermic
True or false: cell store and retrieve energy by making and breaking chemical bonds in metabolic reactions
True
What energy is required to start energy-releasing reactions…
Activation Energy
Minimum amount of energy required to start a reaction
Activation Energy
Energy required to destabilize existing bonds and initiate a chemical reaction
Activation Energy
Transition state- high energy, unstable state (an intermediate form between the substrate and the product)
Activation Energy
True or false: Enzymes function by reducing activation energy of a chemical reaction.
True
Low energy reactants to High energy products=
Endergonic
Reactants stabilize, then peak, then drop to Products=
Exergonic
Substances that influence chemical bonds in a way that LOWERS activation energy
Catalysts (Enzyme)
Cannot violate laws of Thermodynamics
Catalysts
Cannot make an endergonic reaction spontaneous
Catalysts
Does not alter the proportion of reactants turned into product
Catalysts
True or false: Most enzymes are proteins (-ase)
True
True or false: Shape of enzyme stabilizes a temporary association between substates
True
True or false: enzymes are not changed or consumed in the reaction
True
- 200 molecules of carbonic acid per hour made without enzyme
-600,000 molecules formed per second with enzyme
Carbonic Anhydrase
True or false: and enzymes particular substrates bind at its active sites
True
Molecules specifically acted upon by an enzyme
Substrate
Pocket (domain) in an enzyme where substrates bind and a reaction occurs
Active Site
True or false: Enzymes are very substrate specific (fits like a lock & key)
True
True or false: During Enzymatic Reaction, the enzyme is not altered
True
Both enzyme & substrate undergo dynamic conformational changes upon binding to create an enzyme- substrate complex with the best fit.
Induced- Fit
Each enzyme works best within a characteristic range of:
Optimal temp , pH and salt concentration
When Optimal conditions are not met with an enzyme, hydrogen bonds break, the enzyme changes shape & stops working. This process is called?
Denaturation (the enzyme denatures).
Pigment production by the enzyme, Tyrosinase (temp sensitive)
Siamese cat; Micha- Seal Point (coloration of fur)
What type of reactions break down?
Catabolic reactions
What type of reactions release energy?
Exergonic reactions
What type of reactions build up?
Anabolic reactions
What type of reactions store energy?
Endergonic reactions
Net input of energy
Photosynthesis (dehydration)
Net release of energy
Combustion of fuel (hydrolysis)
True or false: Control over enzyme allows cells to conserve energy and resources by producing only what they require
True
True or false: during the control of enzyme activity homeostasis is maintained
True
4 mechanisms of enzyme control
Feedback inhibition, Competitive inhibition, Allosteric inhibition (non competitive), Allosteric activation
Mechanism that slows or stops reactions because of some other activity in the overall pathway
Feedback inhibition
Competes with substrate for active site; Bonds to active site without producing result
Competitive inhibitor
Binds to enzyme at a site other than active site; causes shape change that makes enzyme unable to bind substrate
Allosteric (non-competitive) inhibitor
A region of an enzyme, other than the active site, that can bind regulatory molecules
Allosteric site
Binds to enzyme at a sight other than the active site; causes a mishappen active site to change to an effective active shape
Allosteric activator
Enzyme- mediated reactions, by which cells build; remodel or break down an organic molecules
Metabolic Pathways
Optimal conditions for an enzyme to function:
Under conditions similar to those found in the human body.
Process by which cells derive energy from glucose.
Cellular Respiration
Two mechanisms of ATP synthase
Substrate level phosphorization and oxidative phosphorylization