Chapter 33 - Chromatography

Question 1

What is chromatography?

Answer 1

Chromatography allows different components of mixtures to be seperated and identified depending on the compounds present

Question 2

What advanced types of chromatography exist?

Answer 2

Thin layer chromatography (TLC) in which a TLC plate is coated in a solid which moves up the plate with a solvent (An offshoot of this is HP-TLC)
Column chromatography - A column is packed with a solid and the solvent instead moves down the column
Gas chromatography - Where a column is packed with a solid or with a solid coated by a liquid and a gas is passed through at high temp and pressure

Question 3

What are teh two major factors which affect the movement of the solute?

Answer 3

The retention factor of the stationary phase and how well each compound “sticks” to it
The solubility of the solute in the mobile phase, and how well it absorbs each of the compounds

Question 4

What does the combination of each of these factors result in?

Answer 4

The Rf value of each compound, or the Retardation Factor which is unique to each compound when used with each solvent and stationary phase

Question 5

How do you calculate Rf value?

Answer 5

distance moved by solute / distance moved by solvent

Question 6

How can the components seperated by GC (gas chromatography) be seperated?

Answer 6

By using mass spectrometry