Chapter 3: Observing microorganisms

Question 1

Microorganisms are measured in:

Answer 1

Micrometers and nanometers.
1 micrometer=10^(-6) m
1 nm = 10^(-9) m

Question 2

Five types of light microscopy:

Answer 2

1. ) compound light
2. ) darkfield
3. ) phase-contrast
4. ) fluorescence
5. ) confocal

Question 3

Resolution

Answer 3

The ability to see fine details and structures.

The shorter wavelengths of light provide greater resolution.

Question 4

Refractive index

Answer 4

The measurement ability of the light bending of a medium.

Question 5

Two different electron microscopy types

Answer 5

Transmission EM

scanning EM

Question 6

Chromophore

Answer 6

Stains consist of a positive and negative ion one of which is colored

Question 7

Two types of staining techniques:

Answer 7

Simple and gram

Question 8

Acidic dyes

Answer 8

Negative charged ion chromophore

Question 9

Basic dye

Answer 9

Positive charged ion chromophore

Question 10

Bacterial cell wall is _______ charged.

Answer 10

Negatively

Question 11

Gram stain

Answer 11

Classifies bacteria into gram positive or gram negative

Question 12

Endospore

Answer 12

Resistant dormant structures inside some cells them to not be stained by ordinary methods

Question 13

Color endospores appear

Answer 13

Green within red or pink

Question 14

Biofilm

Answer 14

A community of micro organisms attached to a surface