Chapter 3 - Microscopes Flashcards
What is the refractive index?
The refractive index measures the ability of a medium to bend light
What is the relationship between wavelength and resolution?
The shorter the wavelength, the greater the resolution.
Brightfield microscope
Brightfield microscope uses Visible light to observe stained specimens
Darkfield
Darkfield Uses special condenser to block light to examine living organisms that are invisible in Brightfield or do not stain easily.
Phase-contrast Microscope
Phase-contrast Microscope direct and reflected rays are brought together by annular diaphragm to give a detailed structure of the inside of a cell
Differential interference contrast Microscope
DIC Microscope Uses difference in refractive indexes to provide 3D images.
Flouresence Microscope
Flouresence Microscope Uses UV light to rapidly detect microbes.
Confocal microscope
Confocal microscope Uses single photons to illuminate one plane at a time.
Two-photon microscope
Two-photon microscope Uses 2 photons to illuminate living cells.
Scanning acoustic Microscope
Scanning acoustic Microscope Uses a sound wave to examine living cells attached to another surface.
Transmission electron Microscope
Transmission electron Microscope Transmission Electron Microscope uses a beam of electrons to pass through a specimen to examine viruses.
Scanning electron Microscope
Scanning electron Microscope uses a beam of electrons to study the surface of cells.
Sanning tunneling microscope
Scanning tunneling microscopes use a thin metal probe to produce detailed views of molecules inside a cell.
Atomic Force Microscope
Atomic Force Microscopes use a metal and diamond probe to provide a 3D image of biological specimens.
What is a differential stain?
A differential stain reacts differently with with different kinds of bacteria (gram & acid fast)
