Chapter 3 - Cell Structure Flashcards
What structures are included in the eukaryotic cells for animals?
- nucleus
- mitochondria
- rough endoplasmic reticulum (RER)
- smooth endoplasmic reticulum (SER)
- golgi apparatus
- lysomes
- ribosomes
- cell surface membrane
Describe the nucleus and its function
- nuclear envelope surrounding chromatin + nucleoplasm
* FUNCTION: stores genetic info to make protein and makes ribosome
Describe the mitochondria and its function
- double membrane
- inner is highly folded (cristae)
- FUNCTION: synthesis energy rich ATP molecules during aerobic respiration
Describe the rough endoplasmic reticulum and its function
- network of interjoined sheets studded with ribosomes
* FUNCTION: ribosomes synthesis proteins and channels transport these proteins
Describe the smooth endoplasmic reticulum and its function
- Soft surface and tubular appearance
* FUNCTION: synthesis, store and transports lipids
Describe the golgi apparatus and its function
- from RER then excretes to cell-membrane
* FUNCTION: Modifies proteins or lipids and packages them into vesicles for export
Describing lysosome and its function
- contains hydroletic enzymes
* FUNCTION: Fuses worn out organelles/enzymes digest organelles
Describe ribosomes and its function
- Contains rRNA and protein
- Occurs in cytoplasm or RER
- FUNCTION: Makes proteins
Describe cell-surface membrane and its function
- fluid Mosaic model
* FUNCTION:Controls the entry and exit
Calculate magnification
Image/actual (IAM)
What structures are included in eukaryotic cells for plants?
- cell wall
- chloroplast
- Vacule
Describe a cell wall and its function
- polysaccharides (cellulose)
- thin layer called middle lamellar makes boundary between adjacent cells and cernents adjacent cells
- FUNCTION: mechanical strength to prevent bursting of cell allowing only water to pass
Describe chloroplasts and its function
- chloroplast envelope with double plasma membrane and highly selective
- FUNCTION: harvesting sun light - carries out photosynthesis
Describe a vacule and its function
•FUNCTION: stores H2O
What is a transmission electron microscope?
Passes electrons through specimen giving 2-D images
What is the scanning electron microscope?
Passes electron over the surface of specimens given 3-D imaging (low resolution)
What is an advantage of electron microscope?
High resolving power as electrons have a shorter wavelength than light microscopes
What are disadvantages of a lecture microscopes?
- Systems must have a vacuum - Cannot observe living specimen
- complex staining – yet picture not coloured
- specimen must be thin
- may contain artefact - From the way specimen prepared changing appearance
- expensive
Describe the process of ultracentrifugation
•tissue homogenised in a blender in a solution which is: cold (inactivate enzyme)/isotonic (stops gaining/losing water)/buffered(keep the pH the same)
•tube span at high speed (1000 revs min^-1)
•pellet 1 - nucleus
•supernate removed (all except nuclei)
•run at higher speed
•pellet 2 - chloroplast/mitochondria
Repeat
What’s the fluid left after the cells are broken up by homogeniser releasing organelles from cell?
Homogenate
The two types of electron microscopes?
- Transmission electron microscope
* Scanning electron microscope
When using a light microscope we can measure the size of objects using…………….
An eyepiece graticule
To calibrate an eyepiece graticule you need to use a special microscope slide called a ……………..
Stage micrometer
Describe cell specialisation
It’s cell adapted to have a particular function and perform it to make it more efficient
Describe a tissue
A collection of similar cells for a specific function