Chapter 2 - Nucleic Acids Flashcards
What is the structure of a nucleotide?
•phosphate group
•pentose sugar
•nitrogenous bases (C/A/T/G/U)
These three components join together by condensation reaction forming a mononucleotide and two molecules of water
What does a mononucleotide +mononucleotide form?
Dinucleotide + H20
What bond does a dinucleotide have?
Phosphodiester bond
Complete the sentence:
Polynucleotide forms ……………….
The bases of DNA
What is the purine?
- Guanine
* Adenine
What is a pyrimidine?
- cytosine
- thymine
- uracil
What base goes with cytosine?
Guanine
What base goes with adenine?
Thymine (uracil)
Describe a ribonucleic acid (RNA)
- polymer of nucleotides
- ribo sugar attaches to phosphate group and nucleotide
- contains A/U/C/G
- NO T
- shorter than DNA
What are the three types of RNA? And describe each type
- mRNA - messenger RNA carries DNA code from nucleus to ribose
- tRNA - transfer RNA carries amino acid across cytoplasm
- rRNA - ribosomal RNA makes up tinosomes
Describe a deoxyribonueic acid (DNA)
- Made up of deoxyribose/phosphate group/nucleotide bases
- contains A/T/C/G
- made up of two strands
- longer than RNA
Describe complimentary base pairing
•To DNA polynucleotide strands join by hydrogen bonds •can only pair to specific -A/T (two hydrogen bonds) -G/C (three hydrogen bonds) •always equal amounts
Describe how the DNA is stable
- Phosphodiester backbone protects the more chemically reactive organic bases - You would have to And why the double helix to expose the DNA
- The hydrogen bonds form bridges between nucleotide bases
- There are three hydrogen bonds between C and G – the more C and G bonds the more stable helix
- Anti-parallel arrangement - 5’ to 3’ - nucleic acids can only synthesised in this direction
What are the two main stages of cell division?
- nuclear division
* cytokinesis
Before the first stage of cell division what takes place?
Semi-conservative replication
What is semi-conservative replication?
DNA makes exact copies of itself by undermining the double helix so that each chain acts as a template for the next.
DNA Replication
What are the four requirements for DNA replication?
- Four types of nucleotides (constant supply)
- Strands to act as a template
- DNA polymerase-> catalyse reaction
- source of chemical energy
Describe the process of DNA replication
- Enzyme DNA helicase breaks down hydrogen bonds linking pairs
- As a result double helix unwinds into two strands
- each exposed polynucleotide strands acts as a template to which complimentary nucleotides are attracted
- Energy used to activate these nucleotides
- activated nucleotides are joined together by enzyme DNA polymerases
- each of the new DNA molecules contain one of the original DNA strands
What is pentose sugar is found in DNA?
Deoxyribose
What is the nitrogen and carbon containing molecules compromising to ring structures?
Purine
What is the nitrogen and carbon containing molecules containing molecule comprising a one ring structure?
Pyrimidine
What are the strong bonds formed between adjacent to nucleotides in a polynucleotide?
Phosphodiester
What is the general name for five carbon sugar?
Pentose
What are the following enzymes used for?
•DNA Polymerase:
•DNA Helicase:
- joins the free nucleotides together forming hydrogen bonds
* breaks the hydrogen bonds