Chapter 3 - Cell structure Flashcards
What is an object?
The material placed under the microscope
What is an image?
The appearance of the material viewed under the microscope
Why is the resolution of an electron microscope greater than that of an optical microscope?
Electrons have a very small wavelength compared to light
Equation for magnification
Magnification = size of image/size of real object
What is magnification?
How much bigger the image is than the specimen you’re looking at
What is resolution?
How well a microscope can distinguish between two points that are close together
What do optical microscopes use to form an image?
Light
What do electron microscopes use to form an image?
Electrons
Maximum resolution of a light microscope
0.2 micrometres
Maximum magnification of a light microscope
1500 x
Maximum resolution of electron microscopes
0.0002 micrometres
Maximum magnifiation of electron microscopes
1,500,000 x
What are the two types of electron microscope?
Transmission electron microscope (TEM)
Scanning electron microscope (SEM)
How do transmission electron microscopes work?
A beam of electrons is focussed onto a specimen using electromagnets. The electrons are transmitted THROUGH the specimen. Denser parts of the specimen absorb more electrons, so they appear darker
What are the main limitations of the TEM? (3)
They can only be used on thin specimens
The whole system must be observed in a vacuum - living specimens can’t be viewed
A complex preparation process means artefacts are common
What are artefacts?
Things that you can see on the microscope but aren’t part of your specimen
How many micrometres are in a millimetre?
1,000
How many nanometres are in a micrometre?
1,000
How do scanning electron microscopes work?
A beam of electrons is scanned onto the surface of a specimen. This knocks electrons off of the specimen which are gathered in a cathode ray tube to form an image
Advantages of TEM
High resolution images mean you can see internal structures like chloroplasts
Advantages of SEM
They can be used on thick, and living, specimens
Disadvantages of SEM
The resolution is lower than TEM
What is an eyepiece graticule?
A transparent ruler with numbers but no units
How to calibrate a microscope?
1) Divide total length of stage micrometre by how many intervals to find one micrometre unit
2) Find a point where the two lines are equal
3) Work out how many micrometre units equal how many eyepiece units
4) Convert the stage micrometre units to micrometres
5) Divide stage micrometre units by eyepiece units to find the value of one eyepiece unit
6) This can be used in calculations
What is cell fractionation?
The process where cells are broken up and different organelles they contain are separated out
During homogenisation, why is the solution:
a) cold?
b) the same water potential as the tissue?
c) buffered?
a) to reduce enzyme aactivity that could break down organelles
b) to prevent organelles bursting or shrivelling due to osmosis
c) any change in pH could affect the organelles or enzymes
What happens during homogenisation?
Cells are broken up in a blender to release organelles
What happens during filtration?
The homogenate is filtered to remove any large sections of cells and debris
What happens during ultracentrifugation?
The filtrate is poured into a test tube, placed in the centrifuge and spun slowly
The heaviest organelles sink to the bottom, forming the pellet, whilst the rest of the organelles stay suspended in a fluid called the supernatant
The supernatant is drained off, poured into another test tube and spun at a higher speed
The next heaviest organelles form the pellet and are removed
The process repeats until the desired organelle forms the pellet, which can then be studied
*Description of the nucleus
A large organelle, which is surrounded by the nuclear envelope (a double membrane), covered in pores to allow mRNA to leave. Inside the nucleus is nucleoplasm and in this are chromosomes, containing DNA. One or more nucleoli can also be found her, which are used for making ribosomes.
*Function of the nucleus
The nucleus controls the activities of the cell by controlling the transcription of DNA
*Description of mitochondria
Mitochondria have a double membrane, the innermost of which is folded to form structures called cristae. Surrounding these cristae is a matrix
*Function of cristae
Large surface area for aerobic respiration
*Function of matrix
Contains enzymes involved in respiration
*Function of mitochondria
The site of aerobic respiration, which produces ATP
*Description of cell surface membrane
The membrane found on the surface of animal cells
*Function of cell surface membrane
Controls what enters and leaves cell
*Rough size of mitochondria
Between 2 and 10 um
*What is the chloroplast envelope?
A double membrane surrounding the organelle
*What are grana?
Stacks of thylakoid membranes inside chloroplasts
*What is the stroma?
A matrix where the sugars are synthesised during photosynthesis
*Function of chloroplasts
Site of photosynthesis
*How are chloroplasts adapted for photosynthesis?
The grana provide a large surface area for the first stage of photosynthesis
Chloroplasts contain ribosomes and DNA so they can quickly make proteins required for photosynthesis
What are the two types of endoplasmic reticulum?
Smooth endoplasmic reticulum (SER)
Rough endoplasmic reticulum (SER)
What is the RER?
A system of membranes covered in ribosomes