CHAPTER 3

Question 1

[RESDES]
1st paragraph
Isolation of Pansit-Pansitan will be done by manually drying, powderizing, and cold maceration. Ethanolic extract will be produced from adding 200 mL of 70% ethanol to the chosen amount of powder.

Answer 1

Ling et al., 2020

Question 2

[PHASE 2 - PP]
1st paragraph
This will ensure the solvents bond to the dried plant and prevent fungal growth

Answer 2

Ling, 2020

Question 3

[PHASE 2 - PP]
1st paragraph
the optimum air-drying time will be for a week, with parts of the plant well-separated from each other

Answer 3

Hamdoon et al., 2022

Question 4

[PHASE 2 - PP]
2nd paragraph
It will then be sealed with cotton wool, paraffin film, and aluminum foil respectively

Answer 4

Ling, 2021

Question 5

[PHASE 2 - PP]
3rd paragraph
The study will undergo maceration without agitation

Answer 5

Cacique et al., 2020

Question 6

[PHASE 2 - PP]
3rd paragraph
It will be placed in some amber glass air tight condition

Answer 6

Ling, 2020

Question 7

[PHASE 2 - JBR]
1st paragraph
These symptoms mostly include black or dark brown spots originating from the stem of the mango that advances towards the end of the fruit

Answer 7

Galsurker et al., 2018

Question 8

[PHASE 2 - JBR]
2nd paragraph
The mixture will then be autoclaved under 15 psi, for 25 minutes at 121°C. After sterilization, the media will be cooled.

Answer 8

Durgeshlal, 2019

Question 9

[PHASE 2 - JBR]
3rd paragraph
On the fifth day, the fungi are assumed to change into a dark gray or black color

Answer 9

Komy, Ibrahim, Al-Saleh, 2022

Question 10

[PHASE 2 - JBR]
3rd paragraph
They will continue to incubate for 7 days at 25°C. This duration will be enough to ensure hyphae and conidia growth

Answer 10

Koditwakku et al, 2022

Question 11

[PHASE 2 - JBR]
4th paragraph
it must have a longitudinal striation that measures from 22.4±1.6 to 28.7± 2.8 μm in length and 12.8±1.3 to 15.6±2.4 μm in width using an ocular micrometer.

Answer 11

Komy, Ibrahim, & Al-Saleh, 2022

Question 12

[PHASE 2 - JBR]
5th paragraph
After incubation, cultures will be kept in a refrigerator set at 4°C until eventual experimentation

Answer 12

Durgeshlal, 2019

Question 13

[PHASE 3 - CHARAC ETHANOLIC]
1st paragraph
This test will determine the presence of phenolic extracts, alkaloids, saponins, tannins, and flavonoids in the extract

Answer 13

Kwodaga et al., 2020

Question 14

[PHASE 3 - CHARAC IV]
1st paragrapj
The mixture will then be autoclaved under 15 psi, for 25 minutes at 121°C. After the autoclave, 20 mL of the agar mixture will be poured in each petri dish

Answer 14

Durgeshlal, 2019

Question 15

[PHASE 3 - PREP FOR PFT]
1st paragraph
The petri dishes will be sealed with plastic wrap and tape. To ensure the validity of the experiment, this will be done with three replications

Answer 15

Durgeshlal, 2019

Question 16

[PHASE 3 - PFT]
1st paragraph
The petri dishes will be sealed with plastic wrap and tape. To ensure the validity of the experiment, this will be done with three replications

Answer 16

Durgeshlal, 2019

Question 17

[PHASE 3 - INHIBITION OF MG]
1st paragraph
Each replicate’s mycelial growth of Lasiodiplodia theobromae with a vernier caliper.

Answer 17

Jadav and Kadvani, 2019

Question 18

[PHASE 3 - INHIB MG FORMULA]
1st paragraph
Wherein C will be the diameter growth of the mycelium in the controlled variable, and T will be the diameter growth exhibited in the treated specimen

Answer 18

Durgeshlal, 2019

Question 19

[PHASE 3 - COD]
1st paragraph
One-way ANOVA will utilize Duncan’s multiple range (P < 0.5) and the statistical treatment will be done with the use of SPSS software

Answer 19

Yuan et al., 2019