Chapter 27-28 Flashcards
Order of Life
Domain to kingdom to phylum to class to order to family to genus to species
what kind of bonds do eukarya have?
Ester Bonds
Which domain has rRNA loops?
Bacteria
what are monophyletic groups?
A group of organisms that consists of a common ancestor and all of its descendants
what are paraphyletic groups?
Includes the most recent common ancestor of the group, but not all its descendants
what does an archaea’s cell wall contain?
a surface layer of proteins
what kind of lipids do archaea have?
isoprene chains
what is a functional group?
a group of atoms responsible for the characteristic reactions of a particular compound.
what kind of bonds do bacteria have?
ester bonds
what kind of bonds do archaea have?
ether bonds
What can archaea do that other domains can’t?
Live in extreme environments (extremophiles)
what shape is cocci
sphere shaped bacteria
what are chains of cocci called?
streptococci
what are clumps of cocci called?
staphylococci
what shape is bacilli?
rod shaped
what shape are vibrios
comma shaped
Spirochetes
spiral-shaped bacteria that have flexible walls and are capable of movement
spirilli
spiral shaped bacteria; rigid
what controls bacteria cell shape?
cytoskeletal proteins (MreB and Ftz)
what is a biofilm
collection of microbes living on a surface in a complex community
What is quorum sensing?
the ability of bacteria in a biofilm to communicate with each other and coordinate their activities
what is Gram-positive bacteria
Bacteria that have complex cell walls with thick peptidoglycan but with teichoic acids.
what are sugars attached to a protein called?
glycoprotein
When can a bacteria not be phagocytized?
when it has a capsule of mucilage
where does mucilage come from?
secreted by cells
what is a lose mucilage layer called?
slime layer
why can’t encapsulated bacteria be phagocytized
cells are too slippery to be held on to.
what does mucilage allow for cell communication?
allows quorum sensing and community between multiple species of cells.
what is the form of biofilm?
adhesive, oozing, communitive.
what is peptidoglycan?
a network of sugar polymers cross-linked by polypeptides
what do archaea lack in their cell walls?
peptidoglycan
what do bacteria have in their cell walls?
peptidoglycan
what is peptidoglycan called in bacteria?
murein
what is peptidoglycan called in archaea?
psuedomurein
Why are gram positive bacteria more susceptible to antibiotics?
antibiotics interfere with peptidoglycan synthesis
can GPB hold purple dye?
yes, purple dye is held in thick layers.
what does mordant do?
it facilitates further interactions between the primary dye & the cell wall. It will crystalize the dye that has penetrated the microbe.
what is the wall structure of a gram negative bacteria?
thinner peptidoglycan layer, enclosed in a thin outer envelope; envelope is rich with liposaccharides.
what are liposaccharides?
lipids that have polysaccharides covalently attached to them.
are gram negative cell walls sensitive to antibiotics?
Mostly resistant, but impedes secretion of proteins from outside bacteria.
what are teichoic acids?
gram positive possessing and negatively charged.
how do you treat GNB?
Stop GNB growth.
Why can’t you kill GNB?
it will cause shock; high mortality rate.
how is the cell wall constructed?
lattice; NAG sugars form chains and peptides create a bridge; many layers in order to form a wall.
how many cell wall layers in GNB?
few layers but large periplasmic space
how many cell wall layers in GPB?
many layers but small periplasmic space
what is periplasmic space?
Space between outer membrane and inner membrane (including the peptidoglycan and periplasm, gel between membranes)
what do antibiotics do to the cell wall?
break apart peptic chains
are there gaps in cell wall lattice?
yes, for nutrients.
what is motility?
movement
what are endospores?
DNA enclosure of bacteria cells that are released when a bacterial cell dies and breaks down.
what do all cells have on the surfaces?
sugars
what are proteins with a sugar attached called?
glycoproteins
what part of the cell makes contact with other cells?
the sugar
where are sugars in terms of the cell?
on the outside, never on the inside.
What is horizontal gene transfer?
process where an organism receives genetic material from another organism w/o being offspring.
what is transduction?
the process by which a virus transfers genetic material from one bacterium to another.
what is transformation?
process in which one strain of bacteria is changed by a gene or genes from another strain of bacteria.
what is conjugation?
gene transfer through direct contact.
what is vertical evolution?
gene transfer from parent to offspring.
what does smaller cell size allow?
faster cell division, but limits cell storage.
what are the properties of teichoic acids?
-thread together layers of peptidoglycan
-negatively charged –> retains basic dyes
-found in gram +
what are the charge of the heads in a phospholipid bilayer?
Polar, hydrophilic, positive charge.
what are the charge of the tails in phospholipid bilayer?
tails are hydrophobic.
what allows for cell membrane stability?
rigid carbon structures