Chapter 20 (Oxidative Phosphorylation)

Question 1

The free energy released ETC is conserved by the creation of a ___ across the ___ which is an (exer/ender)gonic process

Answer 1

proton gradient
inner mitochondrial membrane
endergonic

Question 2

Pumping 1H+ out takes about ___kJ/mol, the release of 2e- from NADH generates about ___kJ/mol so NADH can release ___ H+

Answer 2

20
200
10 (from 200/20)

Question 3

The dissipation of the proton gradient is an (exer/ender)gonic process

Answer 3

exergonic

Question 4

ATP is synthesized via the enzyme ___

Answer 4

ATP synthase (Complex V)

Question 5

ATP-ADP exchange

Answer 5

ADP and Pi will become ATP without a proton gradient however it will break back down
We need the proton gradient to release the ATP so it will not breakdown

Question 6

(T/F) ATP synthase can run in both directions

Answer 6

True

Question 7

ATP-synthase is also called

Answer 7

F1-F0-ATPase

Question 8

F0 subunit

Answer 8

Water insoluble transmembrane (bound in membrane) proton channel
Contains the rotor

Question 9

F1 subunit

Answer 9

Water soluble peripheral membrane

Contains the Rotor shaft and Stator

Question 10

The (F1/F0) subunit contains greek letters

Answer 10

F1

Question 11

The ___ subunit is a rotor that turns in the membrane with respect to the ___ subunit

Answer 11

c
a
(refers to F0 because of normal alphabet)

Question 12

Protons entering the a-subunit inlet channel are transferred from an ___ to an ___ located at the midpoint of a ___ subunit

Answer 12

Asn
Asp
c

Question 13

The c subunit rotates ___ to bury the protonated ___ into the hydrophobic membrane

Answer 13

clockwise

Asp

Question 14

The ___ remains protonated until it comes into contact with the ___ subunit outlet channel where the proton is transferred to a ___

Answer 14

Asp
a
Ser

Question 15

pH > pka = (protonated/deprotonated)

Answer 15

deprotonated

Question 16

F1 has (#) catalytic protomers

Answer 16

3 (alpha beta subunits)

Question 17

The ___ and ___ subunits are the only ones that move in ATP-synthase

Answer 17

gamma and c

Question 18

The gamma subunit of ___ is (hydrophobilc/philic)

Answer 18

F1

hydrophobic (makes turning smoother)

Question 19

L

Answer 19

not catalytically active, binds ADP and Pi

Question 20

T

Answer 20

catalytically active, binds ATP

Question 21

O

Answer 21

a low affinity for substrate

Question 22

The conversion of L to T results in:

Answer 22

synthesis of ATP

Question 23

The conversion of T to O results in:

Answer 23

release of ATP

Question 24

___ ATP are synthesized per one complete turn of the ___

Answer 24

3

gamma subunit/ c ring

Question 25

The # of subunits in the c-ring vary from ___ (vertebrates) to ___ (prokaryotes)

Answer 25

8

15

Question 26

It requires ___ protons per ATP synthesized

Answer 26

3

Question 27

P/O ratio for NADH

Answer 27

2.5

Question 28

P/O ratio for FADH2

Answer 28

1.5

Question 29

High [ATP] or low [ADP] leads to (increased/decreased) respiration

Answer 29

decreased

Question 30

Electrons will only flow to O2 when there is a supply of ___

Answer 30

ADP

Question 31

We need both ___ and ___ for ATP synthesis to occur

Answer 31

e- and ADP

Question 32

Uncouplers

Answer 32

inhibit the phosphorylation of ADP without affecting e- transport
(ex. 2,4-dinitrophenol)

Question 33

Uncouplers are (hydrophobilc/philic) molecules with a ___ proton

Answer 33

hydrophobic

dissociable

Question 34

Oxygen consumption is tied to ___ dissipation

Answer 34

gradient